miRNA: 3'- gGUUa---AUcUCAUGGuuGGUACGGUUG- -5'
miRNA: 3'- -GGUuaAU-CUcaUGGUUGGUACGGUUG- -5'
miRNA: 3'- -GGUUAAUCUCAUGGUUGGUACGGUUG- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15218 | 5' | -45.9 | NC_003805.1 | + | 6154 | 0.66 | 0.629596 |
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Target: 5'- aCGAgacgUGuAGUAUCggUCAUGCUAACa -3' miRNA: 3'- gGUUa---AUcUCAUGGuuGGUACGGUUG- -5' |
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| 15218 | 5' | -45.9 | NC_003805.1 | + | 6233 | 0.69 | 0.443168 |
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Target: 5'- cCUAcgUGuGAc-ACCAACCAUGUCAGCa -3' miRNA: 3'- -GGUuaAU-CUcaUGGUUGGUACGGUUG- -5' |
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| 15218 | 5' | -45.9 | NC_003805.1 | + | 1707 | 1.14 | 0.000228 |
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Target: 5'- uCCAAUUAGAGUACCAACCAUGCCAACg -3' miRNA: 3'- -GGUUAAUCUCAUGGUUGGUACGGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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