miRNA: 3'- -AAUAUUUAUu--UUGUgauuGGUCgUCG- -5'
miRNA: 3'- aAUAUUUAUuU-----------UGUGAu---UG-GUCGUCG- -5'
miRNA: 3'- aaUAUuuAUUUUGUGAUUGgU-CGUCG- -5'
miRNA: 3'- aauaUUUAUUuUGUgaUUGGUCGUCG- -5'
miRNA: 3'- -AAUAUUUAUUUUGUGAUUGGUCGUCG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15225 | 3' | -39.1 | NC_003808.1 | + | 876 | 0.68 | 0.773789 |
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Target: 5'- gUUAUGAGUAuauGACAau--CCAGgAGCa -3' miRNA: 3'- -AAUAUUUAUu--UUGUgauuGGUCgUCG- -5' |
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| 15225 | 3' | -39.1 | NC_003808.1 | + | 396 | 0.68 | 0.759226 |
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Target: 5'- cUAUAAAUAuAauccgcuucacACACUcgauGCgCAGCAGCg -3' miRNA: 3'- aAUAUUUAUuU-----------UGUGAu---UG-GUCGUCG- -5' |
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| 15225 | 3' | -39.1 | NC_003808.1 | + | 1609 | 0.72 | 0.526849 |
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Target: 5'- -gAUGuuUGGGACACUGACgAuGCGGUa -3' miRNA: 3'- aaUAUuuAUUUUGUGAUUGgU-CGUCG- -5' |
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| 15225 | 3' | -39.1 | NC_003808.1 | + | 2485 | 0.77 | 0.271954 |
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Target: 5'- ---cAAAUGAcGCAaaAGCCAGCGGCg -3' miRNA: 3'- aauaUUUAUUuUGUgaUUGGUCGUCG- -5' |
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| 15225 | 3' | -39.1 | NC_003808.1 | + | 3262 | 1.13 | 0.000719 |
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Target: 5'- uUUAUAAAUAAAACACUAACCAGCAGCu -3' miRNA: 3'- -AAUAUUUAUUUUGUGAUUGGUCGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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