Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1523 | 3' | -52.1 | NC_001335.1 | + | 17093 | 0.7 | 0.634926 |
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Target: 5'- gUCGUGCCGAUcaacguagCGACCugagucaggaUCGGACCCa-- -3' miRNA: 3'- -AGCAUGGUUA--------GCUGG----------AGCUUGGGcau -5' |
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| 1523 | 3' | -52.1 | NC_001335.1 | + | 45222 | 0.7 | 0.634926 |
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Target: 5'- gUCGgacuucGCCGAcagCGGCCcgaCGAACCCGUAg -3' miRNA: 3'- -AGCa-----UGGUUa--GCUGGa--GCUUGGGCAU- -5' |
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| 1523 | 3' | -52.1 | NC_001335.1 | + | 31684 | 0.7 | 0.631553 |
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Target: 5'- cUCGUGCCAGcgGGCCUUGAugacguaccgccacGCCCGa- -3' miRNA: 3'- -AGCAUGGUUagCUGGAGCU--------------UGGGCau -5' |
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| 1523 | 3' | -52.1 | NC_001335.1 | + | 50641 | 0.71 | 0.601235 |
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Target: 5'- aCGUACCcGUUG-CCUCGAGCgaCGUAg -3' miRNA: 3'- aGCAUGGuUAGCuGGAGCUUGg-GCAU- -5' |
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| 1523 | 3' | -52.1 | NC_001335.1 | + | 46401 | 0.72 | 0.545751 |
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Target: 5'- cUCGcACCAAgaaCGucaaGCCUCGGACCCGg- -3' miRNA: 3'- -AGCaUGGUUa--GC----UGGAGCUUGGGCau -5' |
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| 1523 | 3' | -52.1 | NC_001335.1 | + | 45899 | 0.73 | 0.470168 |
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Target: 5'- -gGUGCCGAacucuuucgcgucUCGGCCUUGAACUCGg- -3' miRNA: 3'- agCAUGGUU-------------AGCUGGAGCUUGGGCau -5' |
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| 1523 | 3' | -52.1 | NC_001335.1 | + | 20967 | 0.73 | 0.460959 |
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Target: 5'- aCGUGCCGAcgCGAUCUCcGAuccagcaguGCCCGUAg -3' miRNA: 3'- aGCAUGGUUa-GCUGGAG-CU---------UGGGCAU- -5' |
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| 1523 | 3' | -52.1 | NC_001335.1 | + | 7209 | 0.74 | 0.421235 |
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Target: 5'- cCGUAUUGAggcagCGACCUCGAGCCCc-- -3' miRNA: 3'- aGCAUGGUUa----GCUGGAGCUUGGGcau -5' |
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| 1523 | 3' | -52.1 | NC_001335.1 | + | 48750 | 1.08 | 0.002599 |
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Target: 5'- cUCGUACCAAUCGACCUCGAACCCGUAg -3' miRNA: 3'- -AGCAUGGUUAGCUGGAGCUUGGGCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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