miRNA: 3'- -GUAGuAC-UCUUCACGCGCUUca-CCUA- -5'
miRNA: 3'- -GUAGUACUCUUCACGCGCUUCACCUA- -5'
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15270 | 5' | -50.6 | NC_003840.1 | + | 3052 | 0.71 | 0.158031 |
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Target: 5'- aCAUCuUGcAGAAGUGCGUGAAuggGGAUa -3' miRNA: 3'- -GUAGuAC-UCUUCACGCGCUUca-CCUA- -5' |
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| 15270 | 5' | -50.6 | NC_003840.1 | + | 3054 | 1.06 | 0.000301 |
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Target: 5'- aCAUCAUGAGAAGUGCGCGAAGUGGAUu -3' miRNA: 3'- -GUAGUACUCUUCACGCGCUUCACCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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