Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
15276 | 3' | -64.3 | NC_003840.1 | + | 761 | 0.66 | 0.050515 |
Target: 5'- cCUUCCCCCUCCUCCUCCuccuucUGCu- -3' miRNA: 3'- -GAAGGGGGGGGGGGGGGuacu--GCGuu -5' |
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15276 | 3' | -64.3 | NC_003840.1 | + | 216 | 0.67 | 0.04165 |
Target: 5'- --gCCCCCCCUCCUgCCAaGACaCAGg -3' miRNA: 3'- gaaGGGGGGGGGGGgGGUaCUGcGUU- -5' |
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15276 | 3' | -64.3 | NC_003840.1 | + | 891 | 0.75 | 0.006707 |
Target: 5'- uCUUUUCCCUCCCCCCCUuUGGCGUg- -3' miRNA: 3'- -GAAGGGGGGGGGGGGGGuACUGCGuu -5' |
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15276 | 3' | -64.3 | NC_003840.1 | + | 516 | 1.05 | 0.000012 |
Target: 5'- uCUUCCCCCCCCCCCCCCAUGACGCAAa -3' miRNA: 3'- -GAAGGGGGGGGGGGGGGUACUGCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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