Results 1 - 20 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
153 | 3' | -49.5 | AC_000007.1 | + | 11432 | 0.66 | 0.916086 |
Target: 5'- aCUUUGAgCCCGacGCGCGGAccgGGAUuaguCCc -3' miRNA: 3'- -GAAACU-GGGCcuUGCGCCU---UUUGu---GG- -5' |
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153 | 3' | -49.5 | AC_000007.1 | + | 18965 | 0.66 | 0.909322 |
Target: 5'- ---cGuCUCGGuGGCGCGGGcaaacuGCACCa -3' miRNA: 3'- gaaaCuGGGCC-UUGCGCCUuu----UGUGG- -5' |
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153 | 3' | -49.5 | AC_000007.1 | + | 25728 | 0.66 | 0.909322 |
Target: 5'- --gUGACCCcaaAGCgGCGGAGGGCAUUc -3' miRNA: 3'- gaaACUGGGcc-UUG-CGCCUUUUGUGG- -5' |
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153 | 3' | -49.5 | AC_000007.1 | + | 25522 | 0.66 | 0.90225 |
Target: 5'- ---cGGCCaCGGAGCGCucguuGAAgGCCg -3' miRNA: 3'- gaaaCUGG-GCCUUGCGccu--UUUgUGG- -5' |
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153 | 3' | -49.5 | AC_000007.1 | + | 12109 | 0.66 | 0.90225 |
Target: 5'- aCUUUGACgCGG-GCGCuGAccuGCGCUg -3' miRNA: 3'- -GAAACUGgGCCuUGCGcCUuu-UGUGG- -5' |
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153 | 3' | -49.5 | AC_000007.1 | + | 5470 | 0.66 | 0.897861 |
Target: 5'- --gUGGCCCuuGGCGCGcagcuugcccuuggaGGAGGCGCCg -3' miRNA: 3'- gaaACUGGGccUUGCGC---------------CUUUUGUGG- -5' |
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153 | 3' | -49.5 | AC_000007.1 | + | 9706 | 0.66 | 0.894874 |
Target: 5'- ---cGAUCCGGucGAUGCGGAcu-CGCUc -3' miRNA: 3'- gaaaCUGGGCC--UUGCGCCUuuuGUGG- -5' |
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153 | 3' | -49.5 | AC_000007.1 | + | 26582 | 0.66 | 0.894874 |
Target: 5'- ----cGCUCGuGGCGCGGGcacaaGAACGCCa -3' miRNA: 3'- gaaacUGGGCcUUGCGCCU-----UUUGUGG- -5' |
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153 | 3' | -49.5 | AC_000007.1 | + | 9245 | 0.67 | 0.887198 |
Target: 5'- ---aGGCCaUGGAGCGCcuugaggccuuGGggGAUAUCa -3' miRNA: 3'- gaaaCUGG-GCCUUGCG-----------CCuuUUGUGG- -5' |
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153 | 3' | -49.5 | AC_000007.1 | + | 27989 | 0.67 | 0.887198 |
Target: 5'- ------aCCGGAGcCGCGGGcaAAGCACUu -3' miRNA: 3'- gaaacugGGCCUU-GCGCCU--UUUGUGG- -5' |
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153 | 3' | -49.5 | AC_000007.1 | + | 23433 | 0.67 | 0.887198 |
Target: 5'- ---aGACCUGGcuaAACGaGGA--GCACCg -3' miRNA: 3'- gaaaCUGGGCC---UUGCgCCUuuUGUGG- -5' |
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153 | 3' | -49.5 | AC_000007.1 | + | 3734 | 0.67 | 0.876781 |
Target: 5'- -cUUGACCUacgagaccgugucuGGAACGCcguuGGAGACugCa -3' miRNA: 3'- gaAACUGGG--------------CCUUGCGc---CUUUUGugG- -5' |
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153 | 3' | -49.5 | AC_000007.1 | + | 5315 | 0.67 | 0.870972 |
Target: 5'- --aUGACCCuGGccCGUcuGGAcGAGCACCg -3' miRNA: 3'- gaaACUGGG-CCuuGCG--CCU-UUUGUGG- -5' |
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153 | 3' | -49.5 | AC_000007.1 | + | 5383 | 0.67 | 0.869286 |
Target: 5'- ----aGCCCGGAGCGCaccccuuCACCg -3' miRNA: 3'- gaaacUGGGCCUUGCGccuuuu-GUGG- -5' |
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153 | 3' | -49.5 | AC_000007.1 | + | 12174 | 0.67 | 0.853628 |
Target: 5'- ---gGACCUGGGcugGCGGu-GGCACCc -3' miRNA: 3'- gaaaCUGGGCCUug-CGCCuuUUGUGG- -5' |
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153 | 3' | -49.5 | AC_000007.1 | + | 6021 | 0.67 | 0.853628 |
Target: 5'- ----cACCCGGucACGUGGccuACACCu -3' miRNA: 3'- gaaacUGGGCCu-UGCGCCuuuUGUGG- -5' |
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153 | 3' | -49.5 | AC_000007.1 | + | 361 | 0.68 | 0.844558 |
Target: 5'- aCUUUGACCguuuACGUGGAGAcuCGCCc -3' miRNA: 3'- -GAAACUGGgccuUGCGCCUUUu-GUGG- -5' |
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153 | 3' | -49.5 | AC_000007.1 | + | 35871 | 0.68 | 0.835237 |
Target: 5'- --gUGGCgCGGGGCGUGGGAAcgGgGCg -3' miRNA: 3'- gaaACUGgGCCUUGCGCCUUU--UgUGg -5' |
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153 | 3' | -49.5 | AC_000007.1 | + | 67 | 0.68 | 0.835237 |
Target: 5'- --gUGGCgCGGGGCGUGGGAAcgGgGCg -3' miRNA: 3'- gaaACUGgGCCUUGCGCCUUU--UgUGg -5' |
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153 | 3' | -49.5 | AC_000007.1 | + | 32665 | 0.68 | 0.825675 |
Target: 5'- --aUGGuCCUGGGAaaGUGGAAAauACACCa -3' miRNA: 3'- gaaACU-GGGCCUUg-CGCCUUU--UGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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