Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
15338 | 3' | -58.6 | NC_003876.1 | + | 1008 | 0.7 | 0.038683 |
Target: 5'- aCGCUGuaacucgcggcgucCACGCCGGUGUGucaGugGUCGCc -3' miRNA: 3'- -GUGACu-------------GUGUGGCCGCAC---CugCGGCG- -5' |
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15338 | 3' | -58.6 | NC_003876.1 | + | 1051 | 1.11 | 0.000007 |
Target: 5'- cCACUGACACACCGGCGUGGACGCCGCg -3' miRNA: 3'- -GUGACUGUGUGGCCGCACCUGCGGCG- -5' |
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15338 | 3' | -58.6 | NC_003876.1 | + | 3831 | 0.68 | 0.057442 |
Target: 5'- uCAUgGAgACACCGGCacGGGCGCgaGCg -3' miRNA: 3'- -GUGaCUgUGUGGCCGcaCCUGCGg-CG- -5' |
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15338 | 3' | -58.6 | NC_003876.1 | + | 2438 | 0.66 | 0.07276 |
Target: 5'- gUAC-GAcCGCACCGGCaauccUGGGCGCgCGUu -3' miRNA: 3'- -GUGaCU-GUGUGGCCGc----ACCUGCG-GCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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