miRNA: 3'- uGGGgGGCAG-AGGCGuuGUGGGgCCG- -5'
miRNA: 3'- -UGGGGgGCA-GAGGc----GUUGuGGG-GCCG- -5'
miRNA: 3'- ugGGGggCAGagGCGUUG-------UGGGGCCG- -5'
miRNA: 3'- ugGGGGGCAGaGGC----GUUGUGG--GGcCG- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1537 | 3' | -62.9 | NC_001347.2 | + | 132670 | 0.66 | 0.738839 |
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Target: 5'- uCUUgCCGUCgUCCGU--CACCCgGGCg -3' miRNA: 3'- uGGGgGGCAG-AGGCGuuGUGGGgCCG- -5' |
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| 1537 | 3' | -62.9 | NC_001347.2 | + | 128410 | 0.66 | 0.738839 |
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Target: 5'- cGCCCCaCGUcCUCCucuuuCAGC-CCCaCGGCg -3' miRNA: 3'- -UGGGGgGCA-GAGGc----GUUGuGGG-GCCG- -5' |
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| 1537 | 3' | -62.9 | NC_001347.2 | + | 21642 | 0.66 | 0.746911 |
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Target: 5'- -gCCUuuGUCggCGCGGCucuucucGCCCUGGCg -3' miRNA: 3'- ugGGGggCAGagGCGUUG-------UGGGGCCG- -5' |
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| 1537 | 3' | -62.9 | NC_001347.2 | + | 229225 | 0.66 | 0.747804 |
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Target: 5'- -aCCCCCGUCcCCGgcccCAACACCguCCcGCa -3' miRNA: 3'- ugGGGGGCAGaGGC----GUUGUGG--GGcCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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