miRNA: 3'- -UGGGGg---GCAGAGGc---GUUGU---GGgGCCG- -5'
miRNA: 3'- -UGGGgGGCaGa---GGCGU--------UGugGGGC-CG- -5'
miRNA: 3'- ugGGGgGCaGAGGCGU--------UGUGG-GGCCG- -5'
miRNA: 3'- -UGGGGgGCagaGGCG--UUGUGGGG----------CCG- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1537 | 3' | -62.9 | NC_001347.2 | + | 20343 | 0.66 | 0.729794 |
|
Target: 5'- cGCCCCaguuCGUCUCCuaauCGACGuguCCgCGGCg -3' miRNA: 3'- -UGGGGg---GCAGAGGc---GUUGU---GGgGCCG- -5' |
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| 1537 | 3' | -62.9 | NC_001347.2 | + | 2394 | 0.66 | 0.711496 |
|
Target: 5'- cGCCCgCCGcCgaggCCGCGcggcggcuGCugCCCGaGCu -3' miRNA: 3'- -UGGGgGGCaGa---GGCGU--------UGugGGGC-CG- -5' |
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| 1537 | 3' | -62.9 | NC_001347.2 | + | 2080 | 0.69 | 0.552134 |
|
Target: 5'- -aCCUgUGcCUCCGCAgccguacgGCGCCgCCGGCg -3' miRNA: 3'- ugGGGgGCaGAGGCGU--------UGUGG-GGCCG- -5' |
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| 1537 | 3' | -62.9 | NC_001347.2 | + | 1063 | 0.68 | 0.627119 |
|
Target: 5'- aACCCCgCGgg-CCGCcgGGCGCCCCuccgaguagcGGCa -3' miRNA: 3'- -UGGGGgGCagaGGCG--UUGUGGGG----------CCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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