Results 121 - 126 of 126 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 1538 | 5' | -62.1 | NC_001347.2 | + | 87653 | 0.66 | 0.759737 |
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Target: 5'- ---aGCGGCGCGcguGCGGaUCaGGCCCg -3' miRNA: 3'- gaugCGCCGCGCu--UGUCgGGgCCGGG- -5' |
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| 1538 | 5' | -62.1 | NC_001347.2 | + | 136084 | 0.66 | 0.759737 |
|
Target: 5'- -gACG-GGCccGCGGACggaccggcgccgGGCCUCGGCCg -3' miRNA: 3'- gaUGCgCCG--CGCUUG------------UCGGGGCCGGg -5' |
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| 1538 | 5' | -62.1 | NC_001347.2 | + | 41693 | 0.66 | 0.759737 |
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Target: 5'- -aACGUGGCGCGu--GGaUgCCGGCCg -3' miRNA: 3'- gaUGCGCCGCGCuugUC-GgGGCCGGg -5' |
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| 1538 | 5' | -62.1 | NC_001347.2 | + | 15643 | 0.66 | 0.759737 |
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Target: 5'- -gGCGCGGaGCGggUGauuaccGCCCUGGUgCa -3' miRNA: 3'- gaUGCGCCgCGCuuGU------CGGGGCCGgG- -5' |
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| 1538 | 5' | -62.1 | NC_001347.2 | + | 39687 | 0.66 | 0.750836 |
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Target: 5'- gUGCaCGGCGCGuGCuggcgccGCCuuGGCgCCa -3' miRNA: 3'- gAUGcGCCGCGCuUGu------CGGggCCG-GG- -5' |
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| 1538 | 5' | -62.1 | NC_001347.2 | + | 40540 | 0.66 | 0.741844 |
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Target: 5'- gCUGC-CGGUGCGuuccACGGUCCUuGCCUg -3' miRNA: 3'- -GAUGcGCCGCGCu---UGUCGGGGcCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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