Results 121 - 126 of 126 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1538 | 5' | -62.1 | NC_001347.2 | + | 87586 | 0.66 | 0.738224 |
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Target: 5'- --cCGUGGcCGUGAGCAGaccgcggugcacgCCCGuGCCCa -3' miRNA: 3'- gauGCGCC-GCGCUUGUCg------------GGGC-CGGG- -5' |
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| 1538 | 5' | -62.1 | NC_001347.2 | + | 40540 | 0.66 | 0.741844 |
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Target: 5'- gCUGC-CGGUGCGuuccACGGUCCUuGCCUg -3' miRNA: 3'- -GAUGcGCCGCGCu---UGUCGGGGcCGGG- -5' |
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| 1538 | 5' | -62.1 | NC_001347.2 | + | 158548 | 0.67 | 0.723621 |
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Target: 5'- gUACGCGaCGUG-ACGGUCCCGcGUCUc -3' miRNA: 3'- gAUGCGCcGCGCuUGUCGGGGC-CGGG- -5' |
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| 1538 | 5' | -62.1 | NC_001347.2 | + | 144450 | 0.67 | 0.723621 |
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Target: 5'- -cACGgGGgGCG-ACGGCCCCgagGGCg- -3' miRNA: 3'- gaUGCgCCgCGCuUGUCGGGG---CCGgg -5' |
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| 1538 | 5' | -62.1 | NC_001347.2 | + | 119092 | 0.67 | 0.723621 |
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Target: 5'- -gGCGCaGcCGCGcuauGCGGCCC-GGCCUc -3' miRNA: 3'- gaUGCGcC-GCGCu---UGUCGGGgCCGGG- -5' |
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| 1538 | 5' | -62.1 | NC_001347.2 | + | 87744 | 0.67 | 0.677003 |
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Target: 5'- ---aGCGGCcagaccucGcCGAAgGaGCCCUGGCCCa -3' miRNA: 3'- gaugCGCCG--------C-GCUUgU-CGGGGCCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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