Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1539 | 5' | -60.1 | NC_001347.2 | + | 107809 | 0.66 | 0.839941 |
Target: 5'- aUCGCgcuggaggaccUGCUGCCCaugcgacUGGCGUcC-CCGGAg -3' miRNA: 3'- -AGCG-----------ACGACGGG-------GCCGCAaGaGGUCU- -5' |
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1539 | 5' | -60.1 | NC_001347.2 | + | 118521 | 0.66 | 0.816738 |
Target: 5'- -aGCUgGCgcagGCCCuCGGCGUaCUgCAGGc -3' miRNA: 3'- agCGA-CGa---CGGG-GCCGCAaGAgGUCU- -5' |
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1539 | 5' | -60.1 | NC_001347.2 | + | 160741 | 0.66 | 0.799982 |
Target: 5'- gCGCUGgUGCCaggCGGUGcUCaCCAGAu -3' miRNA: 3'- aGCGACgACGGg--GCCGCaAGaGGUCU- -5' |
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1539 | 5' | -60.1 | NC_001347.2 | + | 100881 | 0.67 | 0.791395 |
Target: 5'- gCGCgGCgcaGCgCCGGCcaUUCUCCGGGu -3' miRNA: 3'- aGCGaCGa--CGgGGCCGc-AAGAGGUCU- -5' |
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1539 | 5' | -60.1 | NC_001347.2 | + | 192969 | 0.67 | 0.764883 |
Target: 5'- -aGCgGCgcGCCCCGGCGUUCagUCgCGGc -3' miRNA: 3'- agCGaCGa-CGGGGCCGCAAG--AG-GUCu -5' |
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1539 | 5' | -60.1 | NC_001347.2 | + | 199599 | 0.67 | 0.759459 |
Target: 5'- gCGCUGCUguuaucgucgccaccGCCgCCGGCGccgUUCUCgGGc -3' miRNA: 3'- aGCGACGA---------------CGG-GGCCGC---AAGAGgUCu -5' |
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1539 | 5' | -60.1 | NC_001347.2 | + | 198846 | 0.68 | 0.71867 |
Target: 5'- cCGgaGCaUGCCCCGGUgGUUCUCguuGAg -3' miRNA: 3'- aGCgaCG-ACGGGGCCG-CAAGAGgu-CU- -5' |
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1539 | 5' | -60.1 | NC_001347.2 | + | 56439 | 0.68 | 0.690072 |
Target: 5'- cUCGUUGCUGCCgCGcGCGcca-CCAGAc -3' miRNA: 3'- -AGCGACGACGGgGC-CGCaagaGGUCU- -5' |
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1539 | 5' | -60.1 | NC_001347.2 | + | 192137 | 0.68 | 0.687188 |
Target: 5'- gUCGUUGCcgGUCCCcguuuccuccGGCGUcgcgguuuccgccgUCUCCGGAu -3' miRNA: 3'- -AGCGACGa-CGGGG----------CCGCA--------------AGAGGUCU- -5' |
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1539 | 5' | -60.1 | NC_001347.2 | + | 99363 | 0.71 | 0.567953 |
Target: 5'- -aGCUGCUGCCCCguaaagucaaauggcGGCGUggcgucagUCUCCc-- -3' miRNA: 3'- agCGACGACGGGG---------------CCGCA--------AGAGGucu -5' |
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1539 | 5' | -60.1 | NC_001347.2 | + | 1656 | 0.74 | 0.402898 |
Target: 5'- aCGCUGCUGCCucagCCGGCGcUCcgaCAGAc -3' miRNA: 3'- aGCGACGACGG----GGCCGCaAGag-GUCU- -5' |
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1539 | 5' | -60.1 | NC_001347.2 | + | 225492 | 1.09 | 0.001835 |
Target: 5'- aUCGCUGCUGCCCCGGCGUUCUCCAGAa -3' miRNA: 3'- -AGCGACGACGGGGCCGCAAGAGGUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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