miRNA: 3'- -UUGGUACUGUGUGCGUCUCGCACGAG- -5'
miRNA: 3'- uugGUaCUGugUGCguCUCG--CACGAG- -5'
miRNA: 3'- -UUgGUACUGug--UGCGUCUCGCACg---AG- -5'
miRNA: 3'- uuGGUACUGUGUgCGUC-UCGCaCGAg -5'
miRNA: 3'- uUGGU-ACUGUGugCGUCUCGcACGAG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15392 | 3' | -54.8 | NC_003907.2 | + | 6135 | 1.08 | 0.000818 |
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Target: 5'- uAACCAUGACACACGCAGAGCGUGCUCa -3' miRNA: 3'- -UUGGUACUGUGUGCGUCUCGCACGAG- -5' |
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| 15392 | 3' | -54.8 | NC_003907.2 | + | 34081 | 0.68 | 0.532389 |
|
Target: 5'- --aCAaGACugACGguGAGUuaGUGCUCg -3' miRNA: 3'- uugGUaCUGugUGCguCUCG--CACGAG- -5' |
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| 15392 | 3' | -54.8 | NC_003907.2 | + | 32768 | 0.67 | 0.554626 |
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Target: 5'- gGAgUAUGACuugaACGCAGGGCGUGauggUCg -3' miRNA: 3'- -UUgGUACUGug--UGCGUCUCGCACg---AG- -5' |
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| 15392 | 3' | -54.8 | NC_003907.2 | + | 19524 | 0.67 | 0.588458 |
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Target: 5'- -uCCAUGACACA-GUAGcAGCG-GCUa -3' miRNA: 3'- uuGGUACUGUGUgCGUC-UCGCaCGAg -5' |
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| 15392 | 3' | -54.8 | NC_003907.2 | + | 16568 | 0.66 | 0.634069 |
|
Target: 5'- cACCAcUGACAUggGCAGAGU-UGUUCu -3' miRNA: 3'- uUGGU-ACUGUGugCGUCUCGcACGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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