miRNA: 3'- gauACgUCCGUAgUG---UGCGUGCUACC- -5'
miRNA: 3'- gauaCgUC-CGUAGUGUGCguGCUACC- -5'
miRNA: 3'- gauaCGUCCGUAG-------UGUGCGuGCUACc -5'
miRNA: 3'- -GAUaCGuc-CGUAGUGUGCGUGCUacc -5'
miRNA: 3'- -GAUACGUcCGUAGUGUGCGUGCUACC- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15392 | 5' | -51.5 | NC_003907.2 | + | 40691 | 0.66 | 0.849333 |
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Target: 5'- --uUGaAGGCGUgAUugaACGCAUGGUGGa -3' miRNA: 3'- gauACgUCCGUAgUG---UGCGUGCUACC- -5' |
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| 15392 | 5' | -51.5 | NC_003907.2 | + | 7481 | 0.66 | 0.840326 |
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Target: 5'- ---aGgAGcGCAUCACAUGggUGAUGGa -3' miRNA: 3'- gauaCgUC-CGUAGUGUGCguGCUACC- -5' |
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| 15392 | 5' | -51.5 | NC_003907.2 | + | 1212 | 0.68 | 0.726992 |
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Target: 5'- ---aGCAGGCAUUaguaaauACAUGC-CGAUGa -3' miRNA: 3'- gauaCGUCCGUAG-------UGUGCGuGCUACc -5' |
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| 15392 | 5' | -51.5 | NC_003907.2 | + | 2961 | 0.69 | 0.705841 |
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Target: 5'- aCUA-GCucaGCAUCACACGCAUGAc-- -3' miRNA: 3'- -GAUaCGuc-CGUAGUGUGCGUGCUacc -5' |
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| 15392 | 5' | -51.5 | NC_003907.2 | + | 6101 | 0.97 | 0.013686 |
|
Target: 5'- cCUAUGCA-GCAUCACACGCACGAUGGc -3' miRNA: 3'- -GAUACGUcCGUAGUGUGCGUGCUACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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