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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15393 | 5' | -54.3 | NC_003907.2 | + | 11396 | 0.67 | 0.618166 |
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Target: 5'- uGCACgugAGCAGGCUaucaagGUGCUaGCUGCUAu -3' miRNA: 3'- -UGUGa--UUGUCCGA------CGCGAgUGGUGAU- -5' |
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| 15393 | 5' | -54.3 | NC_003907.2 | + | 29072 | 0.67 | 0.561114 |
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Target: 5'- gACugUAGCuGaGCUGCGCcaagcauagccUCugCACUAc -3' miRNA: 3'- -UGugAUUGuC-CGACGCG-----------AGugGUGAU- -5' |
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| 15393 | 5' | -54.3 | NC_003907.2 | + | 1634 | 0.7 | 0.422245 |
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Target: 5'- -gACUAGCAGGCgcaugguuuaUGUGCUCACUguuaaGCUGc -3' miRNA: 3'- ugUGAUUGUCCG----------ACGCGAGUGG-----UGAU- -5' |
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| 15393 | 5' | -54.3 | NC_003907.2 | + | 6682 | 0.72 | 0.298282 |
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Target: 5'- cACGCUGAUGGGCUaGCGU--ACCGCUGc -3' miRNA: 3'- -UGUGAUUGUCCGA-CGCGagUGGUGAU- -5' |
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| 15393 | 5' | -54.3 | NC_003907.2 | + | 6722 | 1.06 | 0.001128 |
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Target: 5'- cACACUAACAGGCUGCGCUCACCACUAu -3' miRNA: 3'- -UGUGAUUGUCCGACGCGAGUGGUGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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