miRNA: 3'- gGUCUA-CAUGugGGg----CUUCGCA----UGUG- -5'
miRNA: 3'- gguCUACAUGU--GGggCUUCGCAuGUg -5'
miRNA: 3'- -GGucUACAUGUGGGGCuuCGCAUGUG- -5'
miRNA: 3'- -GGUCUACAUGUGGGGCUUCGCAUGUG- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15394 | 3' | -51.6 | NC_003907.2 | + | 29966 | 0.66 | 0.878058 |
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Target: 5'- gCAGGUgGUAUugCCgagcaGAAGCGUgaagACGCa -3' miRNA: 3'- gGUCUA-CAUGugGGg----CUUCGCA----UGUG- -5' |
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| 15394 | 3' | -51.6 | NC_003907.2 | + | 23423 | 0.67 | 0.835117 |
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Target: 5'- --cGAUGUGCAuuCCuuGAAGCGU-CAg -3' miRNA: 3'- gguCUACAUGU--GGggCUUCGCAuGUg -5' |
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| 15394 | 3' | -51.6 | NC_003907.2 | + | 13812 | 0.74 | 0.436448 |
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Target: 5'- aCC--GUGUACGCUUCGggGUGUACAUc -3' miRNA: 3'- -GGucUACAUGUGGGGCuuCGCAUGUG- -5' |
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| 15394 | 3' | -51.6 | NC_003907.2 | + | 13886 | 1.13 | 0.001071 |
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Target: 5'- cCCAGAUGUACACCCCGAAGCGUACACg -3' miRNA: 3'- -GGUCUACAUGUGGGGCUUCGCAUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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