miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
15400 5' -54.1 NC_003907.2 + 12449 0.66 0.754667
Target:  5'- aCCCGCCuugggUugCUUAGGUgucaGGUuCUGCCc -3'
miRNA:   3'- -GGGCGG-----AugGAGUCUA----CCAuGAUGGc -5'
15400 5' -54.1 NC_003907.2 + 29990 0.68 0.597949
Target:  5'- aUUGCCUcugguguagguuccAUCgCAGGUGGUAUUGCCGa -3'
miRNA:   3'- gGGCGGA--------------UGGaGUCUACCAUGAUGGC- -5'
15400 5' -54.1 NC_003907.2 + 10860 0.69 0.590189
Target:  5'- uCCCGCCUACUa-AGAUGGauggaguugauUACU-CCGa -3'
miRNA:   3'- -GGGCGGAUGGagUCUACC-----------AUGAuGGC- -5'
15400 5' -54.1 NC_003907.2 + 31793 0.77 0.193325
Target:  5'- gUCGCCUugCUCaAGGUGGUGCUGaauCCGc -3'
miRNA:   3'- gGGCGGAugGAG-UCUACCAUGAU---GGC- -5'
15400 5' -54.1 NC_003907.2 + 34810 1.12 0.000791
Target:  5'- cCCCGCCUACCUCAGAUGGUACUACCGa -3'
miRNA:   3'- -GGGCGGAUGGAGUCUACCAUGAUGGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.