Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15405 | 3' | -45.7 | NC_003907.2 | + | 21152 | 0.66 | 0.989016 |
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Target: 5'- cAGUggGUGcCAuUGAGUAUGCAAUGCAa -3' miRNA: 3'- cUCG--UAUaGU-ACUCGUACGUUAUGUg -5' |
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| 15405 | 3' | -45.7 | NC_003907.2 | + | 3236 | 0.66 | 0.987268 |
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Target: 5'- cGGGCGUAgacuUGAGCAUGUAAUu--- -3' miRNA: 3'- -CUCGUAUagu-ACUCGUACGUUAugug -5' |
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| 15405 | 3' | -45.7 | NC_003907.2 | + | 24244 | 0.67 | 0.97806 |
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Target: 5'- cGAGCAaaUGUC-UGAGCAgauUG-AGUACGCu -3' miRNA: 3'- -CUCGU--AUAGuACUCGU---ACgUUAUGUG- -5' |
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| 15405 | 3' | -45.7 | NC_003907.2 | + | 13448 | 0.68 | 0.964674 |
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Target: 5'- gGAGUA-GUUAUGAGCAaUGCcauccaacgAGUGCACg -3' miRNA: 3'- -CUCGUaUAGUACUCGU-ACG---------UUAUGUG- -5' |
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| 15405 | 3' | -45.7 | NC_003907.2 | + | 20875 | 0.71 | 0.868503 |
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Target: 5'- -cGC-UGUCAUcGAGUAUGCAGUgaaACACu -3' miRNA: 3'- cuCGuAUAGUA-CUCGUACGUUA---UGUG- -5' |
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| 15405 | 3' | -45.7 | NC_003907.2 | + | 19617 | 0.72 | 0.84053 |
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Target: 5'- cGAGCGU-UCAUcAGCAUGUAAUGCu- -3' miRNA: 3'- -CUCGUAuAGUAcUCGUACGUUAUGug -5' |
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| 15405 | 3' | -45.7 | NC_003907.2 | + | 41936 | 0.75 | 0.696032 |
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Target: 5'- gGAGC--GUCcUGuGUAUGCAGUGCACa -3' miRNA: 3'- -CUCGuaUAGuACuCGUACGUUAUGUG- -5' |
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| 15405 | 3' | -45.7 | NC_003907.2 | + | 27300 | 0.75 | 0.647368 |
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Target: 5'- uGAGCuggacuUGUCcgGUGAGUucGUGCAGUACGCa -3' miRNA: 3'- -CUCGu-----AUAG--UACUCG--UACGUUAUGUG- -5' |
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| 15405 | 3' | -45.7 | NC_003907.2 | + | 2554 | 1.12 | 0.004 |
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Target: 5'- gGAGCAUAUCAUGAGCAUGCAAUACACa -3' miRNA: 3'- -CUCGUAUAGUACUCGUACGUUAUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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