Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
15409 | 3' | -50.6 | NC_003907.2 | + | 9676 | 1.16 | 0.00106 |
Target: 5'- uGGACAUGAUAGACGGACGUAACCCCCg -3' miRNA: 3'- -CCUGUACUAUCUGCCUGCAUUGGGGG- -5' |
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15409 | 3' | -50.6 | NC_003907.2 | + | 7108 | 0.73 | 0.569258 |
Target: 5'- uGGugGUGGUAGA-GGACu--ACCCCUc -3' miRNA: 3'- -CCugUACUAUCUgCCUGcauUGGGGG- -5' |
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15409 | 3' | -50.6 | NC_003907.2 | + | 20231 | 0.7 | 0.747932 |
Target: 5'- uGGGCAUGGacguagagcUGGGCGauGGCGUGcuugguGCCCUCa -3' miRNA: 3'- -CCUGUACU---------AUCUGC--CUGCAU------UGGGGG- -5' |
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15409 | 3' | -50.6 | NC_003907.2 | + | 381 | 0.67 | 0.863795 |
Target: 5'- -cAC-UGGUGGAgGGuACGUAACCCaCUg -3' miRNA: 3'- ccUGuACUAUCUgCC-UGCAUUGGG-GG- -5' |
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15409 | 3' | -50.6 | NC_003907.2 | + | 3953 | 0.66 | 0.921923 |
Target: 5'- gGGAUagGGUGG-UGuuCGUAGCCCCg -3' miRNA: 3'- -CCUGuaCUAUCuGCcuGCAUUGGGGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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