Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
15409 | 5' | -50.5 | NC_003907.2 | + | 9710 | 1.12 | 0.00159 |
Target: 5'- aCCGCAGAUGCAGGAGUUCAUCUACGAu -3' miRNA: 3'- -GGCGUCUACGUCCUCAAGUAGAUGCU- -5' |
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15409 | 5' | -50.5 | NC_003907.2 | + | 29784 | 0.75 | 0.432218 |
Target: 5'- aCCaGCAGGUGCAGGcGacagCAUCUAUGGa -3' miRNA: 3'- -GG-CGUCUACGUCCuCaa--GUAGAUGCU- -5' |
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15409 | 5' | -50.5 | NC_003907.2 | + | 43133 | 0.73 | 0.527552 |
Target: 5'- -aGCAGAUGCAGGuaaUUUGUCUGCa- -3' miRNA: 3'- ggCGUCUACGUCCuc-AAGUAGAUGcu -5' |
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15409 | 5' | -50.5 | NC_003907.2 | + | 8469 | 0.67 | 0.84274 |
Target: 5'- gCGUGGA-GCAGGGGcgagguugcgugcUUgGUCUACGAu -3' miRNA: 3'- gGCGUCUaCGUCCUC-------------AAgUAGAUGCU- -5' |
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15409 | 5' | -50.5 | NC_003907.2 | + | 2246 | 0.67 | 0.877872 |
Target: 5'- gCCGUuguGAUagGCAGuGAGUUUGUCUAUGu -3' miRNA: 3'- -GGCGu--CUA--CGUC-CUCAAGUAGAUGCu -5' |
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15409 | 5' | -50.5 | NC_003907.2 | + | 2584 | 0.66 | 0.900672 |
Target: 5'- cCUGCuAGGUGCAGGuGcacaCAUCgACGAc -3' miRNA: 3'- -GGCG-UCUACGUCCuCaa--GUAGaUGCU- -5' |
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15409 | 5' | -50.5 | NC_003907.2 | + | 45511 | 0.66 | 0.914415 |
Target: 5'- uCCGCAGcccagagcugGCGGGGGUUCcgaguaGUgUAUGAa -3' miRNA: 3'- -GGCGUCua--------CGUCCUCAAG------UAgAUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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