Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1541 | 3' | -60 | NC_001347.2 | + | 182190 | 0.66 | 0.885111 |
Target: 5'- -gGGcCGGCCcucggggugcggGUGGcGGGcuGCGUCGCCu -3' miRNA: 3'- ggUCaGCCGG------------CACCuCUC--CGCAGCGG- -5' |
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1541 | 3' | -60 | NC_001347.2 | + | 153247 | 0.66 | 0.885111 |
Target: 5'- --cGUCGGCCGgc--GAcGGCGguUCGCCg -3' miRNA: 3'- gguCAGCCGGCaccuCU-CCGC--AGCGG- -5' |
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1541 | 3' | -60 | NC_001347.2 | + | 228594 | 0.66 | 0.885111 |
Target: 5'- uCCAGUCagcgaGGCCGUcGcGuuGGCG-CGCCa -3' miRNA: 3'- -GGUCAG-----CCGGCAcCuCu-CCGCaGCGG- -5' |
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1541 | 3' | -60 | NC_001347.2 | + | 174644 | 0.66 | 0.881208 |
Target: 5'- gCGGUCGGucuacgucuacucgcCCGUGGuGGAaucgcuguaccuGGUGUCGCg -3' miRNA: 3'- gGUCAGCC---------------GGCACC-UCU------------CCGCAGCGg -5' |
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1541 | 3' | -60 | NC_001347.2 | + | 178053 | 0.66 | 0.878568 |
Target: 5'- gCAGaUGGaCGUGGGGggacugauccAGGCGUCGgCg -3' miRNA: 3'- gGUCaGCCgGCACCUC----------UCCGCAGCgG- -5' |
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1541 | 3' | -60 | NC_001347.2 | + | 185335 | 0.66 | 0.878568 |
Target: 5'- aUAGUauaGGuCCGUGcGAGcAGGCcGcCGCCg -3' miRNA: 3'- gGUCAg--CC-GGCAC-CUC-UCCG-CaGCGG- -5' |
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1541 | 3' | -60 | NC_001347.2 | + | 225629 | 0.66 | 0.871835 |
Target: 5'- aCAG-CaGUCGUGGA-AGG-GUCGCCa -3' miRNA: 3'- gGUCaGcCGGCACCUcUCCgCAGCGG- -5' |
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1541 | 3' | -60 | NC_001347.2 | + | 684 | 0.66 | 0.871835 |
Target: 5'- cCCAGUccaCGGCCuuccagacugcGUGGcGccaAGGCGgCGCCa -3' miRNA: 3'- -GGUCA---GCCGG-----------CACCuC---UCCGCaGCGG- -5' |
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1541 | 3' | -60 | NC_001347.2 | + | 166619 | 0.66 | 0.871835 |
Target: 5'- aCC-GUCacGCCGUccuccGAGAGGCG-CGCCc -3' miRNA: 3'- -GGuCAGc-CGGCAc----CUCUCCGCaGCGG- -5' |
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1541 | 3' | -60 | NC_001347.2 | + | 162169 | 0.66 | 0.864915 |
Target: 5'- -aAGUCGGCgG-GcGGGGGCGUgauccgaccCGCCa -3' miRNA: 3'- ggUCAGCCGgCaCcUCUCCGCA---------GCGG- -5' |
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1541 | 3' | -60 | NC_001347.2 | + | 75858 | 0.66 | 0.857813 |
Target: 5'- --cGUCGGCCGc---GGGGCGcagCGCCu -3' miRNA: 3'- gguCAGCCGGCaccuCUCCGCa--GCGG- -5' |
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1541 | 3' | -60 | NC_001347.2 | + | 92253 | 0.66 | 0.857813 |
Target: 5'- cCCGca-GGCCGUGGccgccgccauGAGGCG-CGUCa -3' miRNA: 3'- -GGUcagCCGGCACCu---------CUCCGCaGCGG- -5' |
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1541 | 3' | -60 | NC_001347.2 | + | 77270 | 0.66 | 0.857093 |
Target: 5'- -uGG-CGGCCGacGAGAgguugaggaucaaGGCGUCGUCg -3' miRNA: 3'- ggUCaGCCGGCacCUCU-------------CCGCAGCGG- -5' |
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1541 | 3' | -60 | NC_001347.2 | + | 161642 | 0.67 | 0.843083 |
Target: 5'- aCCAuGUCGacccgcGgCGUGGAcgcgccguuGGCGUCGCCu -3' miRNA: 3'- -GGU-CAGC------CgGCACCUcu-------CCGCAGCGG- -5' |
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1541 | 3' | -60 | NC_001347.2 | + | 108156 | 0.68 | 0.769284 |
Target: 5'- gCGG-CGGCCGcaGAG-GGCG-CGCCg -3' miRNA: 3'- gGUCaGCCGGCacCUCuCCGCaGCGG- -5' |
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1541 | 3' | -60 | NC_001347.2 | + | 44089 | 0.68 | 0.760459 |
Target: 5'- gUAGUCGGgaCGgaGGAGAGGCGaUGCa -3' miRNA: 3'- gGUCAGCCg-GCa-CCUCUCCGCaGCGg -5' |
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1541 | 3' | -60 | NC_001347.2 | + | 142998 | 0.69 | 0.705696 |
Target: 5'- gCCGGcggCGGCgGUGGuggaGGAGGUugCGCCg -3' miRNA: 3'- -GGUCa--GCCGgCACC----UCUCCGcaGCGG- -5' |
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1541 | 3' | -60 | NC_001347.2 | + | 147919 | 0.69 | 0.705696 |
Target: 5'- gCGGU-GGCCuccGUGGucgaAGGCGUUGCCa -3' miRNA: 3'- gGUCAgCCGG---CACCuc--UCCGCAGCGG- -5' |
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1541 | 3' | -60 | NC_001347.2 | + | 135538 | 0.7 | 0.677485 |
Target: 5'- uCCGGgacCGauuGCCGUcGAGccgGGGCGUCGCCc -3' miRNA: 3'- -GGUCa--GC---CGGCAcCUC---UCCGCAGCGG- -5' |
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1541 | 3' | -60 | NC_001347.2 | + | 74115 | 0.7 | 0.659455 |
Target: 5'- aCGGUCaGCCGcGGGGuugauacccagcgacGGCGUCGCUc -3' miRNA: 3'- gGUCAGcCGGCaCCUCu--------------CCGCAGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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