Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1541 | 3' | -60 | NC_001347.2 | + | 684 | 0.66 | 0.871835 |
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Target: 5'- cCCAGUccaCGGCCuuccagacugcGUGGcGccaAGGCGgCGCCa -3' miRNA: 3'- -GGUCA---GCCGG-----------CACCuC---UCCGCaGCGG- -5' |
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| 1541 | 3' | -60 | NC_001347.2 | + | 166619 | 0.66 | 0.871835 |
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Target: 5'- aCC-GUCacGCCGUccuccGAGAGGCG-CGCCc -3' miRNA: 3'- -GGuCAGc-CGGCAc----CUCUCCGCaGCGG- -5' |
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| 1541 | 3' | -60 | NC_001347.2 | + | 162169 | 0.66 | 0.864915 |
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Target: 5'- -aAGUCGGCgG-GcGGGGGCGUgauccgaccCGCCa -3' miRNA: 3'- ggUCAGCCGgCaCcUCUCCGCA---------GCGG- -5' |
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| 1541 | 3' | -60 | NC_001347.2 | + | 92253 | 0.66 | 0.857813 |
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Target: 5'- cCCGca-GGCCGUGGccgccgccauGAGGCG-CGUCa -3' miRNA: 3'- -GGUcagCCGGCACCu---------CUCCGCaGCGG- -5' |
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| 1541 | 3' | -60 | NC_001347.2 | + | 39370 | 0.73 | 0.499134 |
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Target: 5'- cCCGGUgGacGCCGUGGcccGGCGUCGUCg -3' miRNA: 3'- -GGUCAgC--CGGCACCucuCCGCAGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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