Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
15411 | 3' | -53.6 | NC_003907.2 | + | 26889 | 0.66 | 0.733593 |
Target: 5'- cAGUgugUGCGCCCAcugcuuccUCAA--GACGGGCAg -3' miRNA: 3'- aUCG---AUGCGGGU--------AGUUgaCUGCCUGU- -5' |
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15411 | 3' | -53.6 | NC_003907.2 | + | 27585 | 0.68 | 0.575131 |
Target: 5'- -cGCUACGUUCAUCAACga--GGACu -3' miRNA: 3'- auCGAUGCGGGUAGUUGacugCCUGu -5' |
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15411 | 3' | -53.6 | NC_003907.2 | + | 25504 | 0.69 | 0.530407 |
Target: 5'- cAGCUACGUCCAcugaugaauGCUGACuggGGACAc -3' miRNA: 3'- aUCGAUGCGGGUagu------UGACUG---CCUGU- -5' |
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15411 | 3' | -53.6 | NC_003907.2 | + | 25946 | 0.7 | 0.476424 |
Target: 5'- aGGUgAUGUCCGUCAGUUGAUGGGCGu -3' miRNA: 3'- aUCGaUGCGGGUAGUUGACUGCCUGU- -5' |
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15411 | 3' | -53.6 | NC_003907.2 | + | 25869 | 1.07 | 0.001372 |
Target: 5'- gUAGCUACGCCCAUCAACUGACGGACAu -3' miRNA: 3'- -AUCGAUGCGGGUAGUUGACUGCCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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