Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
15411 | 5' | -55.7 | NC_003907.2 | + | 17839 | 0.7 | 0.438983 |
Target: 5'- gUgGACCACCUcGauGGUGGGUAUGGg -3' miRNA: 3'- gAgCUGGUGGAaCggUCAUCCGUGCC- -5' |
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15411 | 5' | -55.7 | NC_003907.2 | + | 20209 | 0.74 | 0.251183 |
Target: 5'- aUCGAgUACCUUGaagaagaAGUGGGCAUGGa -3' miRNA: 3'- gAGCUgGUGGAACgg-----UCAUCCGUGCC- -5' |
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15411 | 5' | -55.7 | NC_003907.2 | + | 6960 | 0.75 | 0.197979 |
Target: 5'- --gGGCUACCUUGCCcGUAGGUAUGa -3' miRNA: 3'- gagCUGGUGGAACGGuCAUCCGUGCc -5' |
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15411 | 5' | -55.7 | NC_003907.2 | + | 25905 | 1.1 | 0.000642 |
Target: 5'- cCUCGACCACCUUGCCAGUAGGCACGGu -3' miRNA: 3'- -GAGCUGGUGGAACGGUCAUCCGUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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