Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
15412 | 3' | -54.7 | NC_003907.2 | + | 27630 | 1.12 | 0.000576 |
Target: 5'- uAGCUCCGUAGGCGAGAACACAGUGCCu -3' miRNA: 3'- -UCGAGGCAUCCGCUCUUGUGUCACGG- -5' |
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15412 | 3' | -54.7 | NC_003907.2 | + | 3121 | 0.75 | 0.220837 |
Target: 5'- uAGCUCCGUcacuGGGUGGGGACACcAGUuaCu -3' miRNA: 3'- -UCGAGGCA----UCCGCUCUUGUG-UCAcgG- -5' |
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15412 | 3' | -54.7 | NC_003907.2 | + | 31737 | 0.68 | 0.581748 |
Target: 5'- aAGCUUCGUAuucguGGacaGGGucGCGCAGUGCg -3' miRNA: 3'- -UCGAGGCAU-----CCg--CUCu-UGUGUCACGg -5' |
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15412 | 3' | -54.7 | NC_003907.2 | + | 30241 | 0.66 | 0.68244 |
Target: 5'- cGGUUgUGaaUAGaCGGGGAUGCAGUGCCu -3' miRNA: 3'- -UCGAgGC--AUCcGCUCUUGUGUCACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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