Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
15413 | 3' | -52.8 | NC_003907.2 | + | 6598 | 0.66 | 0.794716 |
Target: 5'- uGCaUCGGgGAGCGUAgugaccaucuGGUCU--GCCCCu -3' miRNA: 3'- -CG-GGUCgUUCGCAU----------UCAGGaaUGGGG- -5' |
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15413 | 3' | -52.8 | NC_003907.2 | + | 10969 | 0.66 | 0.794716 |
Target: 5'- -aCCAGUAA-CGUAAGUUC-UACCgCCu -3' miRNA: 3'- cgGGUCGUUcGCAUUCAGGaAUGG-GG- -5' |
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15413 | 3' | -52.8 | NC_003907.2 | + | 44133 | 0.66 | 0.778455 |
Target: 5'- gGUCCAuugauuggugguaugGguGGCGguGGUCCUgugacUACCCCa -3' miRNA: 3'- -CGGGU---------------CguUCGCauUCAGGA-----AUGGGG- -5' |
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15413 | 3' | -52.8 | NC_003907.2 | + | 9407 | 0.66 | 0.773287 |
Target: 5'- aGCCagAGCAccuAGCGUAAGcgcacccUUCUUACCUg -3' miRNA: 3'- -CGGg-UCGU---UCGCAUUC-------AGGAAUGGGg -5' |
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15413 | 3' | -52.8 | NC_003907.2 | + | 21728 | 0.67 | 0.720829 |
Target: 5'- aCCCAGCAuagcaagguAGCGUAcc-CCUcGCUCCa -3' miRNA: 3'- cGGGUCGU---------UCGCAUucaGGAaUGGGG- -5' |
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15413 | 3' | -52.8 | NC_003907.2 | + | 15677 | 0.7 | 0.541134 |
Target: 5'- uGCCaCAGU--GCGcAAGUCCUUAUCaCCa -3' miRNA: 3'- -CGG-GUCGuuCGCaUUCAGGAAUGG-GG- -5' |
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15413 | 3' | -52.8 | NC_003907.2 | + | 45287 | 0.71 | 0.530149 |
Target: 5'- -gCCAGCGGuCGUAGGuucgaaUCCUacUACCCCg -3' miRNA: 3'- cgGGUCGUUcGCAUUC------AGGA--AUGGGG- -5' |
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15413 | 3' | -52.8 | NC_003907.2 | + | 34025 | 0.72 | 0.435759 |
Target: 5'- aGCacaCAGUGAGCGguGGUCCaUUGCUCCa -3' miRNA: 3'- -CGg--GUCGUUCGCauUCAGG-AAUGGGG- -5' |
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15413 | 3' | -52.8 | NC_003907.2 | + | 31611 | 1.14 | 0.000667 |
Target: 5'- aGCCCAGCAAGCGUAAGUCCUUACCCCc -3' miRNA: 3'- -CGGGUCGUUCGCAUUCAGGAAUGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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