Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15415 | 5' | -50.5 | NC_003907.2 | + | 29964 | 0.66 | 0.855723 |
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Target: 5'- -aGGUGGUAuUGCCGagcagaagcgugaagAcGCAGCGAAg -3' miRNA: 3'- gaCUACCGUuACGGU---------------UaCGUCGCUUg -5' |
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| 15415 | 5' | -50.5 | NC_003907.2 | + | 30463 | 0.66 | 0.842953 |
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Target: 5'- uUGAagaaGCAAUGCCAGUGaCGGCaGAGa -3' miRNA: 3'- gACUac--CGUUACGGUUAC-GUCG-CUUg -5' |
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| 15415 | 5' | -50.5 | NC_003907.2 | + | 37543 | 0.67 | 0.823855 |
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Target: 5'- gCUGGUGGUGAUGCUcGUucuacuaCAGCGggUa -3' miRNA: 3'- -GACUACCGUUACGGuUAc------GUCGCuuG- -5' |
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| 15415 | 5' | -50.5 | NC_003907.2 | + | 11497 | 0.67 | 0.823855 |
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Target: 5'- gUGAUGGCAcccUGCuCAAUcauaGCAGCuAGCa -3' miRNA: 3'- gACUACCGUu--ACG-GUUA----CGUCGcUUG- -5' |
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| 15415 | 5' | -50.5 | NC_003907.2 | + | 6857 | 0.67 | 0.803837 |
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Target: 5'- uCUGGUgGGCuGAUGCUaaGAUGCuacguGUGAACg -3' miRNA: 3'- -GACUA-CCG-UUACGG--UUACGu----CGCUUG- -5' |
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| 15415 | 5' | -50.5 | NC_003907.2 | + | 19190 | 0.69 | 0.693576 |
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Target: 5'- -aGGUGGCAGUGCUcGUGUAcGUGGuACu -3' miRNA: 3'- gaCUACCGUUACGGuUACGU-CGCU-UG- -5' |
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| 15415 | 5' | -50.5 | NC_003907.2 | + | 39416 | 0.7 | 0.670265 |
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Target: 5'- gCUcAUGuGUggUGCCAAUGCAGCa--- -3' miRNA: 3'- -GAcUAC-CGuuACGGUUACGUCGcuug -5' |
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| 15415 | 5' | -50.5 | NC_003907.2 | + | 11799 | 0.75 | 0.382974 |
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Target: 5'- -cGGUGGCuugGUGCagauAUGCGGUGAGCa -3' miRNA: 3'- gaCUACCGu--UACGgu--UACGUCGCUUG- -5' |
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| 15415 | 5' | -50.5 | NC_003907.2 | + | 36903 | 1.03 | 0.004961 |
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Target: 5'- gCUGAUGGCAAUGCCAAUGCAGCGAc- -3' miRNA: 3'- -GACUACCGUUACGGUUACGUCGCUug -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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