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miRNA: 3'- gUGGCGC-ACGUaaUG----UgCGCCUAUGU- -5'
miRNA: 3'- gUGG--CGCa--CGUa----AUGUGCGCC--UAUGU- -5'
miRNA: 3'- gUGgCGCACGuaAUGUGCgccUAUGU- -5'
miRNA: 3'- -GUGGCGCA-CguAAuGUGCGCCuaUGU- -5'
miRNA: 3'- -GUGGCG--CACGUaaUGuGCGC-CUAUgu -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 1542 | 3' | -52.1 | NC_001347.2 | + | 74860 | 0.66 | 0.994402 |
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Target: 5'- gGCCGCGcUGCAccACcucgGgGUGGAUGCGg -3' miRNA: 3'- gUGGCGC-ACGUaaUG----UgCGCCUAUGU- -5' |
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| 1542 | 3' | -52.1 | NC_001347.2 | + | 77769 | 0.66 | 0.995165 |
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Target: 5'- gGCCgaGCGggcGCAauuugUACGCGCGGccGUGCAg -3' miRNA: 3'- gUGG--CGCa--CGUa----AUGUGCGCC--UAUGU- -5' |
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| 1542 | 3' | -52.1 | NC_001347.2 | + | 150922 | 0.66 | 0.993545 |
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Target: 5'- aGCgGCGUGCcgUGCGCGa--AUGCAu -3' miRNA: 3'- gUGgCGCACGuaAUGUGCgccUAUGU- -5' |
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| 1542 | 3' | -52.1 | NC_001347.2 | + | 192650 | 0.66 | 0.992586 |
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Target: 5'- aCGCUGCGUcGguUUcUGCGCGGcgACAg -3' miRNA: 3'- -GUGGCGCA-CguAAuGUGCGCCuaUGU- -5' |
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| 1542 | 3' | -52.1 | NC_001347.2 | + | 40390 | 0.66 | 0.995841 |
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Target: 5'- aCACCGCcgGUGCGggGC-CGCGuGGUGg- -3' miRNA: 3'- -GUGGCG--CACGUaaUGuGCGC-CUAUgu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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