Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1542 | 3' | -52.1 | NC_001347.2 | + | 22502 | 0.71 | 0.92454 |
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Target: 5'- uCACCGUGUGCAcggGCuuuUGCGGAgaaagGCGc -3' miRNA: 3'- -GUGGCGCACGUaa-UGu--GCGCCUa----UGU- -5' |
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| 1542 | 3' | -52.1 | NC_001347.2 | + | 14537 | 0.71 | 0.929853 |
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Target: 5'- uGCCGCGaGCcgUugACGCcguugGGAUACGc -3' miRNA: 3'- gUGGCGCaCGuaAugUGCG-----CCUAUGU- -5' |
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| 1542 | 3' | -52.1 | NC_001347.2 | + | 6092 | 0.68 | 0.97557 |
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Target: 5'- uCACCcCGUGUG-UACACGCcguGGGUGCGu -3' miRNA: 3'- -GUGGcGCACGUaAUGUGCG---CCUAUGU- -5' |
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| 1542 | 3' | -52.1 | NC_001347.2 | + | 1320 | 0.66 | 0.995446 |
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Target: 5'- aCGCCGCGcagccagaugaacgGCGUggGCGCGaCGGAccUGCGu -3' miRNA: 3'- -GUGGCGCa-------------CGUAa-UGUGC-GCCU--AUGU- -5' |
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| 1542 | 3' | -52.1 | NC_001347.2 | + | 813 | 0.71 | 0.907168 |
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Target: 5'- aACCGCGUGCuc-GCugACGCGGGUuuGCu -3' miRNA: 3'- gUGGCGCACGuaaUG--UGCGCCUA--UGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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