Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1543 | 5' | -57.1 | NC_001347.2 | + | 135211 | 0.66 | 0.924826 |
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Target: 5'- aGGCGCcgacCCCGGCUgggcgGCCGaUGGGgggagggGAGUGg -3' miRNA: 3'- -CUGCG----GGGUCGA-----CGGC-AUCCa------CUUAC- -5' |
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| 1543 | 5' | -57.1 | NC_001347.2 | + | 80978 | 0.66 | 0.919431 |
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Target: 5'- cGGCGCCgCC-GUUGCCGUAGaaGAAg- -3' miRNA: 3'- -CUGCGG-GGuCGACGGCAUCcaCUUac -5' |
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| 1543 | 5' | -57.1 | NC_001347.2 | + | 222282 | 0.66 | 0.901889 |
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Target: 5'- ---uCCUCAGCUGCCGUAcGUGAu-- -3' miRNA: 3'- cugcGGGGUCGACGGCAUcCACUuac -5' |
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| 1543 | 5' | -57.1 | NC_001347.2 | + | 37923 | 0.68 | 0.829622 |
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Target: 5'- cGGCGCCCaCAGCgcgGCgCGcGGGUGcaGGUGc -3' miRNA: 3'- -CUGCGGG-GUCGa--CG-GCaUCCAC--UUAC- -5' |
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| 1543 | 5' | -57.1 | NC_001347.2 | + | 85307 | 0.68 | 0.829622 |
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Target: 5'- -uCGCCCgGGCUGCCccgcAGGUG-GUGa -3' miRNA: 3'- cuGCGGGgUCGACGGca--UCCACuUAC- -5' |
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| 1543 | 5' | -57.1 | NC_001347.2 | + | 113181 | 0.72 | 0.612445 |
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Target: 5'- cGCGCCCCAGCcGCCGgc-GUGGcgGc -3' miRNA: 3'- cUGCGGGGUCGaCGGCaucCACUuaC- -5' |
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| 1543 | 5' | -57.1 | NC_001347.2 | + | 214720 | 1.07 | 0.004039 |
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Target: 5'- cGACGCCCCAGCUGCCGUAGGUGAAUGc -3' miRNA: 3'- -CUGCGGGGUCGACGGCAUCCACUUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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