Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
15430 | 3' | -59.8 | NC_003930.1 | + | 5859 | 0.68 | 0.097456 |
Target: 5'- aCUCCgGCUCgcUGGCgGUGgggaGCUCCGCUa -3' miRNA: 3'- -GAGG-CGGGa-ACCGaCACg---CGAGGUGG- -5' |
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15430 | 3' | -59.8 | NC_003930.1 | + | 10304 | 0.68 | 0.094477 |
Target: 5'- --gCGCCCgUGGcCUGUGUGCUCaucauuacauaCGCCc -3' miRNA: 3'- gagGCGGGaACC-GACACGCGAG-----------GUGG- -5' |
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15430 | 3' | -59.8 | NC_003930.1 | + | 8900 | 0.74 | 0.032053 |
Target: 5'- gCUCCGCCCggagaaagagGGCUGUGUcuacgucGCcugCCGCCu -3' miRNA: 3'- -GAGGCGGGaa--------CCGACACG-------CGa--GGUGG- -5' |
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15430 | 3' | -59.8 | NC_003930.1 | + | 3999 | 1.11 | 0.000026 |
Target: 5'- aCUCCGCCCUUGGCUGUGCGCUCCACCc -3' miRNA: 3'- -GAGGCGGGAACCGACACGCGAGGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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