Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15431 | 3' | -56.2 | NC_003930.1 | + | 11609 | 0.67 | 0.210582 |
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Target: 5'- --aGUGCgCUgGUgAGUGCACUCCACCg -3' miRNA: 3'- gccCACGaGAgUG-UCACGUGAGGUGG- -5' |
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| 15431 | 3' | -56.2 | NC_003930.1 | + | 9322 | 0.67 | 0.206876 |
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Target: 5'- cCGGGUGCcaUC-CAGUGUggccgcaacgguuagGCUCUGCCc -3' miRNA: 3'- -GCCCACGagAGuGUCACG---------------UGAGGUGG- -5' |
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| 15431 | 3' | -56.2 | NC_003930.1 | + | 9740 | 0.67 | 0.19845 |
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Target: 5'- aCGGGauccuUGCUUUCACuuccuUGC-CUCCGCUa -3' miRNA: 3'- -GCCC-----ACGAGAGUGuc---ACGuGAGGUGG- -5' |
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| 15431 | 3' | -56.2 | NC_003930.1 | + | 11642 | 0.7 | 0.125551 |
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Target: 5'- uGGaGUGCaCUCAcCAGcGCACUUCACUa -3' miRNA: 3'- gCC-CACGaGAGU-GUCaCGUGAGGUGG- -5' |
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| 15431 | 3' | -56.2 | NC_003930.1 | + | 301 | 0.78 | 0.028534 |
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Target: 5'- uCGGGUGCcccgauucuUCUCGCuGGUGCGCUCC-CUa -3' miRNA: 3'- -GCCCACG---------AGAGUG-UCACGUGAGGuGG- -5' |
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| 15431 | 3' | -56.2 | NC_003930.1 | + | 5285 | 1.12 | 5.5e-05 |
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Target: 5'- gCGGGUGCUCUCACAGUGCACUCCACCu -3' miRNA: 3'- -GCCCACGAGAGUGUCACGUGAGGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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