miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
15432 3' -53.5 NC_003930.1 + 9351 0.66 0.369696
Target:  5'- uGGUGuuCGaaaACCguggUGCAUUGAugCCGGg -3'
miRNA:   3'- -UCAUuuGCg--UGG----ACGUGGCUugGGCC- -5'
15432 3' -53.5 NC_003930.1 + 4409 0.66 0.350422
Target:  5'- --aGAGCGCACCacacaCACCGucaCCGGg -3'
miRNA:   3'- ucaUUUGCGUGGac---GUGGCuugGGCC- -5'
15432 3' -53.5 NC_003930.1 + 5708 0.67 0.341057
Target:  5'- -cUGAAgGCGCCcGCuCUGuGCCCGGu -3'
miRNA:   3'- ucAUUUgCGUGGaCGuGGCuUGGGCC- -5'
15432 3' -53.5 NC_003930.1 + 5414 0.73 0.132105
Target:  5'- ----cGCaCACCUGCGCCGggUUCGGu -3'
miRNA:   3'- ucauuUGcGUGGACGUGGCuuGGGCC- -5'
15432 3' -53.5 NC_003930.1 + 9895 0.74 0.096163
Target:  5'- aGGgcAA-GUACCUGCGCCGcauCCCGGu -3'
miRNA:   3'- -UCauUUgCGUGGACGUGGCuu-GGGCC- -5'
15432 3' -53.5 NC_003930.1 + 8586 0.76 0.07191
Target:  5'- cGGUGAugGC-CCUGcCACUGucACCCGGc -3'
miRNA:   3'- -UCAUUugCGuGGAC-GUGGCu-UGGGCC- -5'
15432 3' -53.5 NC_003930.1 + 5463 1.1 0.000166
Target:  5'- cAGUAAACGCACCUGCACCGAACCCGGc -3'
miRNA:   3'- -UCAUUUGCGUGGACGUGGCUUGGGCC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.