Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15432 | 3' | -53.5 | NC_003930.1 | + | 9351 | 0.66 | 0.369696 |
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Target: 5'- uGGUGuuCGaaaACCguggUGCAUUGAugCCGGg -3' miRNA: 3'- -UCAUuuGCg--UGG----ACGUGGCUugGGCC- -5' |
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| 15432 | 3' | -53.5 | NC_003930.1 | + | 4409 | 0.66 | 0.350422 |
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Target: 5'- --aGAGCGCACCacacaCACCGucaCCGGg -3' miRNA: 3'- ucaUUUGCGUGGac---GUGGCuugGGCC- -5' |
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| 15432 | 3' | -53.5 | NC_003930.1 | + | 5708 | 0.67 | 0.341057 |
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Target: 5'- -cUGAAgGCGCCcGCuCUGuGCCCGGu -3' miRNA: 3'- ucAUUUgCGUGGaCGuGGCuUGGGCC- -5' |
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| 15432 | 3' | -53.5 | NC_003930.1 | + | 5414 | 0.73 | 0.132105 |
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Target: 5'- ----cGCaCACCUGCGCCGggUUCGGu -3' miRNA: 3'- ucauuUGcGUGGACGUGGCuuGGGCC- -5' |
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| 15432 | 3' | -53.5 | NC_003930.1 | + | 9895 | 0.74 | 0.096163 |
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Target: 5'- aGGgcAA-GUACCUGCGCCGcauCCCGGu -3' miRNA: 3'- -UCauUUgCGUGGACGUGGCuu-GGGCC- -5' |
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| 15432 | 3' | -53.5 | NC_003930.1 | + | 8586 | 0.76 | 0.07191 |
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Target: 5'- cGGUGAugGC-CCUGcCACUGucACCCGGc -3' miRNA: 3'- -UCAUUugCGuGGAC-GUGGCu-UGGGCC- -5' |
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| 15432 | 3' | -53.5 | NC_003930.1 | + | 5463 | 1.1 | 0.000166 |
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Target: 5'- cAGUAAACGCACCUGCACCGAACCCGGc -3' miRNA: 3'- -UCAUUUGCGUGGACGUGGCUUGGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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