Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1544 | 3' | -57.1 | NC_001347.2 | + | 79083 | 0.66 | 0.924917 |
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Target: 5'- gCGAAGCGGCGGgCGGagugCCAGa---- -3' miRNA: 3'- gGUUUCGCCGCCaGCCa---GGUCcaguu -5' |
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| 1544 | 3' | -57.1 | NC_001347.2 | + | 136208 | 0.66 | 0.919573 |
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Target: 5'- gCCGGGG-GGCGG-CGGgcacgCCGGGUUu- -3' miRNA: 3'- -GGUUUCgCCGCCaGCCa----GGUCCAGuu -5' |
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| 1544 | 3' | -57.1 | NC_001347.2 | + | 174631 | 0.67 | 0.908216 |
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Target: 5'- cUCAucGGCgaaGGCGGUCGGUCUAcGUCu- -3' miRNA: 3'- -GGUu-UCG---CCGCCAGCCAGGUcCAGuu -5' |
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| 1544 | 3' | -57.1 | NC_001347.2 | + | 173578 | 0.68 | 0.854219 |
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Target: 5'- gCCAAuGcCGGCGGUcugcaugacgaCGGcCCGGGUCu- -3' miRNA: 3'- -GGUUuC-GCCGCCA-----------GCCaGGUCCAGuu -5' |
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| 1544 | 3' | -57.1 | NC_001347.2 | + | 207541 | 0.69 | 0.80914 |
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Target: 5'- aCAuucAAGCGGCaGGUCGaGUuggaggacuugcuacCCAGGUCAc -3' miRNA: 3'- gGU---UUCGCCG-CCAGC-CA---------------GGUCCAGUu -5' |
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| 1544 | 3' | -57.1 | NC_001347.2 | + | 100872 | 0.7 | 0.779394 |
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Target: 5'- gCCGAGGCGGCGcGgcgcagcgcCGGccauucUCCGGGUCGc -3' miRNA: 3'- -GGUUUCGCCGC-Ca--------GCC------AGGUCCAGUu -5' |
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| 1544 | 3' | -57.1 | NC_001347.2 | + | 124197 | 0.74 | 0.55683 |
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Target: 5'- gUCAGAcGCuGGUGaUCGGUCCGGGUCAc -3' miRNA: 3'- -GGUUU-CG-CCGCcAGCCAGGUCCAGUu -5' |
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| 1544 | 3' | -57.1 | NC_001347.2 | + | 210488 | 1.08 | 0.004267 |
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Target: 5'- aCCAAAGCGGCGGUCGGUCCAGGUCAAc -3' miRNA: 3'- -GGUUUCGCCGCCAGCCAGGUCCAGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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