Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
15442 | 3' | -55.1 | NC_003930.1 | + | 1694 | 0.66 | 0.299965 |
Target: 5'- --cGGCGAAGAAGaCGGgGCG-GGUg -3' miRNA: 3'- caaCUGCUUCUUUgGCCgCGCgCCGu -5' |
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15442 | 3' | -55.1 | NC_003930.1 | + | 8999 | 0.66 | 0.291645 |
Target: 5'- -aUGGgGAGGcAGCUGcGUGgGCGGCGa -3' miRNA: 3'- caACUgCUUCuUUGGC-CGCgCGCCGU- -5' |
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15442 | 3' | -55.1 | NC_003930.1 | + | 4587 | 0.68 | 0.187799 |
Target: 5'- -gUGACuAGGGuAGCCGcCGCGCGGUAu -3' miRNA: 3'- caACUGcUUCU-UUGGCcGCGCGCCGU- -5' |
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15442 | 3' | -55.1 | NC_003930.1 | + | 207 | 0.72 | 0.100599 |
Target: 5'- --aGugGGAGAAAgcuCUGGCG-GCGGCAu -3' miRNA: 3'- caaCugCUUCUUU---GGCCGCgCGCCGU- -5' |
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15442 | 3' | -55.1 | NC_003930.1 | + | 1655 | 0.77 | 0.039121 |
Target: 5'- --cGAgGAGGAAGCCGGCGa-CGGCAu -3' miRNA: 3'- caaCUgCUUCUUUGGCCGCgcGCCGU- -5' |
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15442 | 3' | -55.1 | NC_003930.1 | + | 4511 | 1.08 | 0.000126 |
Target: 5'- gGUUGACGAAGAAACCGGCGCGCGGCAg -3' miRNA: 3'- -CAACUGCUUCUUUGGCCGCGCGCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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