Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
15446 | 3' | -61.7 | NC_003930.1 | + | 5356 | 0.67 | 0.081363 |
Target: 5'- gGCUGCCAGuGucGCCGCGAGUCacacagccaGCAGg -3' miRNA: 3'- gCGGCGGUC-Cu-CGGCGUUCAGc--------CGUU- -5' |
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15446 | 3' | -61.7 | NC_003930.1 | + | 10605 | 0.68 | 0.07177 |
Target: 5'- aGCCGCCccauguGGGcuGCUGCAcGUCGGUg- -3' miRNA: 3'- gCGGCGG------UCCu-CGGCGUuCAGCCGuu -5' |
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15446 | 3' | -61.7 | NC_003930.1 | + | 707 | 0.69 | 0.052149 |
Target: 5'- aGCUGCCAGuaccgccGAGUCGCuccAGUUGGCGu -3' miRNA: 3'- gCGGCGGUC-------CUCGGCGu--UCAGCCGUu -5' |
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15446 | 3' | -61.7 | NC_003930.1 | + | 7963 | 0.94 | 0.000388 |
Target: 5'- gCGCCGCCAGGAGCCGCAAGUC-GCAAc -3' miRNA: 3'- -GCGGCGGUCCUCGGCGUUCAGcCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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