Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15448 | 5' | -52.4 | NC_003930.1 | + | 9020 | 0.66 | 0.434945 |
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Target: 5'- -aGcGUGUAUCuCGCCGAuaGCCAUCg -3' miRNA: 3'- gaC-CACAUGGuGUGGUUcgUGGUAGg -5' |
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| 15448 | 5' | -52.4 | NC_003930.1 | + | 7944 | 0.67 | 0.371679 |
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Target: 5'- ----cGUACCggccgcGCACUAAGCGCCG-CCa -3' miRNA: 3'- gaccaCAUGG------UGUGGUUCGUGGUaGG- -5' |
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| 15448 | 5' | -52.4 | NC_003930.1 | + | 10156 | 0.68 | 0.323958 |
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Target: 5'- ----aGUACCGCACCcuGCugCAUgCg -3' miRNA: 3'- gaccaCAUGGUGUGGuuCGugGUAgG- -5' |
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| 15448 | 5' | -52.4 | NC_003930.1 | + | 5763 | 0.68 | 0.314979 |
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Target: 5'- -gGGUGUGCuCACGCUAcuaGGCgGCCcgCUg -3' miRNA: 3'- gaCCACAUG-GUGUGGU---UCG-UGGuaGG- -5' |
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| 15448 | 5' | -52.4 | NC_003930.1 | + | 10055 | 0.7 | 0.242606 |
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Target: 5'- uUGGUGUGgUACACCAgaaguucccaggGGUACgCAUCa -3' miRNA: 3'- gACCACAUgGUGUGGU------------UCGUG-GUAGg -5' |
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| 15448 | 5' | -52.4 | NC_003930.1 | + | 1811 | 0.7 | 0.215152 |
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Target: 5'- --cGUGUugCGCACCAGGaaacugGCCAUUCu -3' miRNA: 3'- gacCACAugGUGUGGUUCg-----UGGUAGG- -5' |
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| 15448 | 5' | -52.4 | NC_003930.1 | + | 9352 | 0.73 | 0.13922 |
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Target: 5'- uUGGUGUucgaaaACCguggugcauugAUGCCGGGUGCCAUCCa -3' miRNA: 3'- gACCACA------UGG-----------UGUGGUUCGUGGUAGG- -5' |
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| 15448 | 5' | -52.4 | NC_003930.1 | + | 4275 | 0.75 | 0.09154 |
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Target: 5'- aUGGUagGUGCCGCACCGGGgUACagaGUCCu -3' miRNA: 3'- gACCA--CAUGGUGUGGUUC-GUGg--UAGG- -5' |
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| 15448 | 5' | -52.4 | NC_003930.1 | + | 10027 | 1.13 | 0.000112 |
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Target: 5'- uCUGGUGUACCACACCAAGCACCAUCCa -3' miRNA: 3'- -GACCACAUGGUGUGGUUCGUGGUAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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