Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1545 | 5' | -59.6 | NC_001347.2 | + | 80493 | 0.66 | 0.846535 |
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Target: 5'- gGCCCUGCUCGg-CCcacacgccguggaaGGGCGcgCAGUc -3' miRNA: 3'- aCGGGGUGAGCgaGG--------------UCCGCuaGUCA- -5' |
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| 1545 | 5' | -59.6 | NC_001347.2 | + | 174610 | 0.66 | 0.844225 |
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Target: 5'- gGCcgCCCAC-CGCUaCCcguacucaucggcgaAGGCGGUCGGUc -3' miRNA: 3'- aCG--GGGUGaGCGA-GG---------------UCCGCUAGUCA- -5' |
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| 1545 | 5' | -59.6 | NC_001347.2 | + | 107405 | 0.66 | 0.841121 |
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Target: 5'- cUGCCUUACUuuguaccgccaCGCUCCGGcGCGG-CGGa -3' miRNA: 3'- -ACGGGGUGA-----------GCGAGGUC-CGCUaGUCa -5' |
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| 1545 | 5' | -59.6 | NC_001347.2 | + | 20393 | 0.67 | 0.800101 |
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Target: 5'- gUGCCCCACUCGCUCgCuuGUGuauaAGa -3' miRNA: 3'- -ACGGGGUGAGCGAG-GucCGCuag-UCa -5' |
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| 1545 | 5' | -59.6 | NC_001347.2 | + | 153805 | 0.67 | 0.764732 |
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Target: 5'- aGUCCgAgUCGcCUCCGGGCGAggUgGGUg -3' miRNA: 3'- aCGGGgUgAGC-GAGGUCCGCU--AgUCA- -5' |
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| 1545 | 5' | -59.6 | NC_001347.2 | + | 167264 | 0.7 | 0.630691 |
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Target: 5'- cGCCCCACUUGCUgugCAGGUcccGAUaCAGg -3' miRNA: 3'- aCGGGGUGAGCGAg--GUCCG---CUA-GUCa -5' |
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| 1545 | 5' | -59.6 | NC_001347.2 | + | 136056 | 0.7 | 0.601291 |
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Target: 5'- gGCCCCGgauggUGCUCCAgGGCGGUgGGa -3' miRNA: 3'- aCGGGGUga---GCGAGGU-CCGCUAgUCa -5' |
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| 1545 | 5' | -59.6 | NC_001347.2 | + | 19636 | 0.71 | 0.572079 |
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Target: 5'- uUGCCgCCAUgUCGCUCC--GCGGUCAGg -3' miRNA: 3'- -ACGG-GGUG-AGCGAGGucCGCUAGUCa -5' |
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| 1545 | 5' | -59.6 | NC_001347.2 | + | 209124 | 1.07 | 0.002464 |
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Target: 5'- uUGCCCCACUCGCUCCAGGCGAUCAGUa -3' miRNA: 3'- -ACGGGGUGAGCGAGGUCCGCUAGUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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