Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1546 | 5' | -52.1 | NC_001347.2 | + | 202309 | 0.66 | 0.996904 |
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Target: 5'- aGUCgCGuGUGuuuugacgGGGCUGGGugGAGAGg -3' miRNA: 3'- -CAGaGC-CACua------CUCGGCUUugCUCUC- -5' |
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| 1546 | 5' | -52.1 | NC_001347.2 | + | 125189 | 0.67 | 0.991418 |
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Target: 5'- -cCUCGGgGAUcagcGAGuCCGAAACcGAGGGa -3' miRNA: 3'- caGAGCCaCUA----CUC-GGCUUUG-CUCUC- -5' |
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| 1546 | 5' | -52.1 | NC_001347.2 | + | 152331 | 0.68 | 0.985856 |
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Target: 5'- ---aCGGUGAUGAugcuGCUGAGACuGAGAc -3' miRNA: 3'- cagaGCCACUACU----CGGCUUUG-CUCUc -5' |
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| 1546 | 5' | -52.1 | NC_001347.2 | + | 8171 | 0.68 | 0.982202 |
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Target: 5'- aGUCaugcCGGUG--GGGCCGGGACGGGGu -3' miRNA: 3'- -CAGa---GCCACuaCUCGGCUUUGCUCUc -5' |
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| 1546 | 5' | -52.1 | NC_001347.2 | + | 62423 | 0.68 | 0.97788 |
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Target: 5'- uUCaagCaGUGGUaGGGcCCGAGACGAGAGg -3' miRNA: 3'- cAGa--GcCACUA-CUC-GGCUUUGCUCUC- -5' |
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| 1546 | 5' | -52.1 | NC_001347.2 | + | 40659 | 0.69 | 0.975449 |
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Target: 5'- ---aCGGUGuugGGGCCGggGaCGGGGGu -3' miRNA: 3'- cagaGCCACua-CUCGGCuuU-GCUCUC- -5' |
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| 1546 | 5' | -52.1 | NC_001347.2 | + | 37801 | 0.74 | 0.826748 |
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Target: 5'- uGUCaugaCGGUGGUGAgGUCGAGAcCGAGAGu -3' miRNA: 3'- -CAGa---GCCACUACU-CGGCUUU-GCUCUC- -5' |
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| 1546 | 5' | -52.1 | NC_001347.2 | + | 165525 | 0.77 | 0.675261 |
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Target: 5'- cGUCUCGGUGGUGccGuuGAAgggccGCGAGAGc -3' miRNA: 3'- -CAGAGCCACUACu-CggCUU-----UGCUCUC- -5' |
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| 1546 | 5' | -52.1 | NC_001347.2 | + | 208787 | 1.1 | 0.009752 |
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Target: 5'- gGUCUCGGUGAUGAGCCGAAACGAGAGg -3' miRNA: 3'- -CAGAGCCACUACUCGGCUUUGCUCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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