Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1547 | 3' | -57.4 | NC_001347.2 | + | 100196 | 0.66 | 0.920151 |
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Target: 5'- gCCGCGGCCucgcccaGGUAGCCgGAgacggCGGu -3' miRNA: 3'- -GGCGCCGG-------UCGUUGGgCUagaa-GCCu -5' |
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| 1547 | 3' | -57.4 | NC_001347.2 | + | 171326 | 0.66 | 0.930928 |
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Target: 5'- -gGCGGCCuGUcuCCCGGcggCUcUCGGAc -3' miRNA: 3'- ggCGCCGGuCGuuGGGCUa--GA-AGCCU- -5' |
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| 1547 | 3' | -57.4 | NC_001347.2 | + | 44382 | 0.66 | 0.932872 |
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Target: 5'- gCCGgGGCUucuggagaacgccggGGCAGCagCGAUCUggGGAa -3' miRNA: 3'- -GGCgCCGG---------------UCGUUGg-GCUAGAagCCU- -5' |
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| 1547 | 3' | -57.4 | NC_001347.2 | + | 78431 | 0.66 | 0.935722 |
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Target: 5'- aCC-CGGcCCGGCGgcGCCCGAg---CGGGa -3' miRNA: 3'- -GGcGCC-GGUCGU--UGGGCUagaaGCCU- -5' |
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| 1547 | 3' | -57.4 | NC_001347.2 | + | 116889 | 0.66 | 0.935722 |
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Target: 5'- cCCGCGcGCCccccgGGCAACCCGccgccggCUaCGGc -3' miRNA: 3'- -GGCGC-CGG-----UCGUUGGGCua-----GAaGCCu -5' |
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| 1547 | 3' | -57.4 | NC_001347.2 | + | 82221 | 0.66 | 0.935722 |
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Target: 5'- uCCGCGGUaugcgugaccaCAcGCGGCUCGugcaCUUCGGGa -3' miRNA: 3'- -GGCGCCG-----------GU-CGUUGGGCua--GAAGCCU- -5' |
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| 1547 | 3' | -57.4 | NC_001347.2 | + | 37077 | 0.66 | 0.939851 |
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Target: 5'- gCCGUGGCCuGUGGCCCGAagauguacacauaUCga-GGu -3' miRNA: 3'- -GGCGCCGGuCGUUGGGCU-------------AGaagCCu -5' |
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| 1547 | 3' | -57.4 | NC_001347.2 | + | 105989 | 0.66 | 0.940299 |
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Target: 5'- gCGCGGCCgcAGCAcgugucGCCCGAcgacgagaUCgcgCGGc -3' miRNA: 3'- gGCGCCGG--UCGU------UGGGCU--------AGaa-GCCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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