Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1548 | 5' | -56.1 | NC_001347.2 | + | 75314 | 0.66 | 0.959211 |
Target: 5'- uUCCAucCGUugagauacCGGCACGCUCUG--GCCGc -3' miRNA: 3'- cAGGU--GCA--------GUCGUGCGAGACacUGGC- -5' |
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1548 | 5' | -56.1 | NC_001347.2 | + | 202051 | 0.66 | 0.955575 |
Target: 5'- -gCCAacaaUCAGCACGUUUgcaucGUGGCCGa -3' miRNA: 3'- caGGUgc--AGUCGUGCGAGa----CACUGGC- -5' |
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1548 | 5' | -56.1 | NC_001347.2 | + | 32020 | 0.66 | 0.951726 |
Target: 5'- aUCUGCGgUGGCACGC-CUGUGuACCc -3' miRNA: 3'- cAGGUGCaGUCGUGCGaGACAC-UGGc -5' |
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1548 | 5' | -56.1 | NC_001347.2 | + | 93121 | 0.66 | 0.947659 |
Target: 5'- -aCCACGUCAGCGuaccaagucUGUUCggcgGgcgGACCGc -3' miRNA: 3'- caGGUGCAGUCGU---------GCGAGa---Ca--CUGGC- -5' |
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1548 | 5' | -56.1 | NC_001347.2 | + | 38852 | 0.67 | 0.938862 |
Target: 5'- cGUCCGCGUC-GCGCGCcgCUagGAUCa -3' miRNA: 3'- -CAGGUGCAGuCGUGCGa-GAcaCUGGc -5' |
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1548 | 5' | -56.1 | NC_001347.2 | + | 58861 | 0.67 | 0.929169 |
Target: 5'- uUCCAcCGgcagCGGCACGC-CggaGUGACCu -3' miRNA: 3'- cAGGU-GCa---GUCGUGCGaGa--CACUGGc -5' |
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1548 | 5' | -56.1 | NC_001347.2 | + | 63113 | 0.67 | 0.929169 |
Target: 5'- -gCCGcCG-CGGC-CGCcgCUGUGGCCGg -3' miRNA: 3'- caGGU-GCaGUCGuGCGa-GACACUGGC- -5' |
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1548 | 5' | -56.1 | NC_001347.2 | + | 22198 | 0.67 | 0.929169 |
Target: 5'- cUCCAuCG-CAGcCACGCgCUgGUGGCCGa -3' miRNA: 3'- cAGGU-GCaGUC-GUGCGaGA-CACUGGC- -5' |
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1548 | 5' | -56.1 | NC_001347.2 | + | 1834 | 0.68 | 0.900372 |
Target: 5'- cGUUCGCGUCGGCGCcgcaccccgcgucGCUgCUGacGGCCGu -3' miRNA: 3'- -CAGGUGCAGUCGUG-------------CGA-GACa-CUGGC- -5' |
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1548 | 5' | -56.1 | NC_001347.2 | + | 208008 | 0.68 | 0.89469 |
Target: 5'- -aCCGCGUCuGGUAcCGCUaCUGUGGgCGa -3' miRNA: 3'- caGGUGCAG-UCGU-GCGA-GACACUgGC- -5' |
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1548 | 5' | -56.1 | NC_001347.2 | + | 210236 | 0.68 | 0.88817 |
Target: 5'- cGUCCGCGUCucguGCAUcCUCUGccGGCUGc -3' miRNA: 3'- -CAGGUGCAGu---CGUGcGAGACa-CUGGC- -5' |
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1548 | 5' | -56.1 | NC_001347.2 | + | 185562 | 0.68 | 0.881438 |
Target: 5'- cGUCCGCaGUCGG-GCGaaaUCUGUGuACCGu -3' miRNA: 3'- -CAGGUG-CAGUCgUGCg--AGACAC-UGGC- -5' |
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1548 | 5' | -56.1 | NC_001347.2 | + | 142133 | 0.69 | 0.867348 |
Target: 5'- -aCCGCGUCAuGCugGCgCUGgacGCCGa -3' miRNA: 3'- caGGUGCAGU-CGugCGaGACac-UGGC- -5' |
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1548 | 5' | -56.1 | NC_001347.2 | + | 165512 | 0.69 | 0.844729 |
Target: 5'- cGUCCAgGUC-GCACGuCUCgGUGguGCCGu -3' miRNA: 3'- -CAGGUgCAGuCGUGC-GAGaCAC--UGGC- -5' |
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1548 | 5' | -56.1 | NC_001347.2 | + | 226757 | 0.71 | 0.775999 |
Target: 5'- gGUCgGCGUCGGCAUGCcgCUgaucgcaGUGGCCc -3' miRNA: 3'- -CAGgUGCAGUCGUGCGa-GA-------CACUGGc -5' |
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1548 | 5' | -56.1 | NC_001347.2 | + | 96938 | 0.72 | 0.711016 |
Target: 5'- cGUCCGCGcCuGCACGCcgCcGUGACUGc -3' miRNA: 3'- -CAGGUGCaGuCGUGCGa-GaCACUGGC- -5' |
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1548 | 5' | -56.1 | NC_001347.2 | + | 15079 | 0.72 | 0.691493 |
Target: 5'- cGUCCGCGacCGGgACGCaguUUUGUGGCCGa -3' miRNA: 3'- -CAGGUGCa-GUCgUGCG---AGACACUGGC- -5' |
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1548 | 5' | -56.1 | NC_001347.2 | + | 174802 | 0.72 | 0.691493 |
Target: 5'- aGUUCAUGUUGGCGCGCgacCUGcUGGCCu -3' miRNA: 3'- -CAGGUGCAGUCGUGCGa--GAC-ACUGGc -5' |
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1548 | 5' | -56.1 | NC_001347.2 | + | 206087 | 1.1 | 0.004005 |
Target: 5'- cGUCCACGUCAGCACGCUCUGUGACCGg -3' miRNA: 3'- -CAGGUGCAGUCGUGCGAGACACUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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