Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1549 | 3' | -51.4 | NC_001347.2 | + | 149287 | 0.66 | 0.997702 |
Target: 5'- uGUGGAUCCgCUGACGCguuUGG--UCAUCGa -3' miRNA: 3'- -CAUCUAGG-GGUUGCG---GCCaaAGUAGU- -5' |
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1549 | 3' | -51.4 | NC_001347.2 | + | 29242 | 0.66 | 0.996248 |
Target: 5'- -cGGAUCgagugCCGACGCCGGUggaggaCGUCu -3' miRNA: 3'- caUCUAGg----GGUUGCGGCCAaa----GUAGu -5' |
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1549 | 3' | -51.4 | NC_001347.2 | + | 133467 | 0.67 | 0.993214 |
Target: 5'- aUAGGUgCCgGACGCCGGcuaCGUCc -3' miRNA: 3'- cAUCUAgGGgUUGCGGCCaaaGUAGu -5' |
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1549 | 3' | -51.4 | NC_001347.2 | + | 301 | 0.67 | 0.993214 |
Target: 5'- --cGGUCCCCAGgGCCG---UCGUCc -3' miRNA: 3'- cauCUAGGGGUUgCGGCcaaAGUAGu -5' |
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1549 | 3' | -51.4 | NC_001347.2 | + | 229780 | 0.67 | 0.993214 |
Target: 5'- --cGGUCCCCAGgGCCG---UCGUCc -3' miRNA: 3'- cauCUAGGGGUUgCGGCcaaAGUAGu -5' |
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1549 | 3' | -51.4 | NC_001347.2 | + | 85393 | 0.68 | 0.986959 |
Target: 5'- cGUGGcGUCCCCGuCGCUGuugUCGUCAc -3' miRNA: 3'- -CAUC-UAGGGGUuGCGGCcaaAGUAGU- -5' |
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1549 | 3' | -51.4 | NC_001347.2 | + | 38189 | 0.69 | 0.970591 |
Target: 5'- --cGGUCCCCGACGCCGccggacccgcCAUCGc -3' miRNA: 3'- cauCUAGGGGUUGCGGCcaaa------GUAGU- -5' |
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1549 | 3' | -51.4 | NC_001347.2 | + | 204917 | 1.09 | 0.011302 |
Target: 5'- cGUAGAUCCCCAACGCCGGUUUCAUCAu -3' miRNA: 3'- -CAUCUAGGGGUUGCGGCCAAAGUAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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