Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1550 | 3' | -44 | NC_001347.2 | + | 40397 | 0.66 | 1 |
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Target: 5'- -----cGGUGCGGgGCCgcgugGUGggUCCu -3' miRNA: 3'- uauuauCUAUGCCaUGGa----CACuaAGG- -5' |
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| 1550 | 3' | -44 | NC_001347.2 | + | 228355 | 0.67 | 1 |
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Target: 5'- gGUGGUGcGAUGCGGUGgaUGUGGcggggugcggcgguUUCCg -3' miRNA: 3'- -UAUUAU-CUAUGCCAUggACACU--------------AAGG- -5' |
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| 1550 | 3' | -44 | NC_001347.2 | + | 24521 | 0.71 | 0.999909 |
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Target: 5'- ----gAGGUGCuccuccuGGUGCCUGUGGccUCCg -3' miRNA: 3'- uauuaUCUAUG-------CCAUGGACACUa-AGG- -5' |
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| 1550 | 3' | -44 | NC_001347.2 | + | 59459 | 0.73 | 0.999493 |
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Target: 5'- -aGGUGGAUGCGGUGCg-GUGucgCCu -3' miRNA: 3'- uaUUAUCUAUGCCAUGgaCACuaaGG- -5' |
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| 1550 | 3' | -44 | NC_001347.2 | + | 206731 | 0.73 | 0.999034 |
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Target: 5'- -aGGUAGAUguuuGCGGcGCCUGUGGUaaaCCg -3' miRNA: 3'- uaUUAUCUA----UGCCaUGGACACUAa--GG- -5' |
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| 1550 | 3' | -44 | NC_001347.2 | + | 204076 | 1.11 | 0.049661 |
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Target: 5'- uAUAAUAGAUACGGUACCUGUGAUUCCc -3' miRNA: 3'- -UAUUAUCUAUGCCAUGGACACUAAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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