Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1551 | 3' | -55.9 | NC_001347.2 | + | 171955 | 0.66 | 0.963269 |
Target: 5'- aGUCGGGUUUGCCGcgcUGcGAGugguUCGAa -3' miRNA: 3'- aCGGCCUAGACGGCu--AC-CUCu---AGCUg -5' |
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1551 | 3' | -55.9 | NC_001347.2 | + | 139412 | 0.66 | 0.963269 |
Target: 5'- uUGCCGG-UCaGCUGAuuccaugcgUGGAGGaggCGACc -3' miRNA: 3'- -ACGGCCuAGaCGGCU---------ACCUCUa--GCUG- -5' |
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1551 | 3' | -55.9 | NC_001347.2 | + | 187 | 0.66 | 0.95987 |
Target: 5'- gUGgCGGGUgUGCCGgcGGGGuguggUGGCg -3' miRNA: 3'- -ACgGCCUAgACGGCuaCCUCua---GCUG- -5' |
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1551 | 3' | -55.9 | NC_001347.2 | + | 229666 | 0.66 | 0.95987 |
Target: 5'- gUGgCGGGUgUGCCGgcGGGGuguggUGGCg -3' miRNA: 3'- -ACgGCCUAgACGGCuaCCUCua---GCUG- -5' |
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1551 | 3' | -55.9 | NC_001347.2 | + | 198132 | 0.66 | 0.956261 |
Target: 5'- cGCgCGGuggcagCgggGgUGGUGGGGAUCGGCa -3' miRNA: 3'- aCG-GCCua----Ga--CgGCUACCUCUAGCUG- -5' |
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1551 | 3' | -55.9 | NC_001347.2 | + | 136106 | 0.66 | 0.956261 |
Target: 5'- cGCCGGGccUCgGCCGG-GGAGggUGGg -3' miRNA: 3'- aCGGCCU--AGaCGGCUaCCUCuaGCUg -5' |
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1551 | 3' | -55.9 | NC_001347.2 | + | 178906 | 0.66 | 0.951248 |
Target: 5'- cGCCGGucguggugaaggauGUCguguacacgGCCGG-GGAGggCGACg -3' miRNA: 3'- aCGGCC--------------UAGa--------CGGCUaCCUCuaGCUG- -5' |
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1551 | 3' | -55.9 | NC_001347.2 | + | 108306 | 0.66 | 0.944132 |
Target: 5'- cGCCGGugCUGCCGcacGAGAcgCGACu -3' miRNA: 3'- aCGGCCuaGACGGCuacCUCUa-GCUG- -5' |
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1551 | 3' | -55.9 | NC_001347.2 | + | 135965 | 0.67 | 0.934932 |
Target: 5'- gGCCGGG-CcGUCGGauggGGGGGUCGAg -3' miRNA: 3'- aCGGCCUaGaCGGCUa---CCUCUAGCUg -5' |
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1551 | 3' | -55.9 | NC_001347.2 | + | 136184 | 0.67 | 0.918324 |
Target: 5'- gGCCGGAaccCUGCCGcggacugcgccGGGGggCGGCg -3' miRNA: 3'- aCGGCCUa--GACGGCua---------CCUCuaGCUG- -5' |
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1551 | 3' | -55.9 | NC_001347.2 | + | 47104 | 0.67 | 0.913809 |
Target: 5'- uUGUCGGccgacaUGCCGAUGGuauGggCGGCg -3' miRNA: 3'- -ACGGCCuag---ACGGCUACCu--CuaGCUG- -5' |
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1551 | 3' | -55.9 | NC_001347.2 | + | 69837 | 0.68 | 0.907961 |
Target: 5'- cGCCGuuGUCU-CCGGcUGGAGAUCaGACg -3' miRNA: 3'- aCGGCc-UAGAcGGCU-ACCUCUAG-CUG- -5' |
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1551 | 3' | -55.9 | NC_001347.2 | + | 190153 | 0.68 | 0.907961 |
Target: 5'- -aCCGGGuUCUGCCGGcaUGGGGggCGcCa -3' miRNA: 3'- acGGCCU-AGACGGCU--ACCUCuaGCuG- -5' |
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1551 | 3' | -55.9 | NC_001347.2 | + | 113045 | 0.71 | 0.786715 |
Target: 5'- cGCCGccUCUGCCGGcuccgagGGAGAaagCGACg -3' miRNA: 3'- aCGGCcuAGACGGCUa------CCUCUa--GCUG- -5' |
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1551 | 3' | -55.9 | NC_001347.2 | + | 143670 | 0.75 | 0.553103 |
Target: 5'- cGCCGGAg--GCCGGccUGGAGGUCuGCg -3' miRNA: 3'- aCGGCCUagaCGGCU--ACCUCUAGcUG- -5' |
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1551 | 3' | -55.9 | NC_001347.2 | + | 203439 | 1.1 | 0.003724 |
Target: 5'- uUGCCGGAUCUGCCGAUGGAGAUCGACa -3' miRNA: 3'- -ACGGCCUAGACGGCUACCUCUAGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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