Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15513 | 5' | -41.9 | NC_004001.2 | + | 18927 | 0.66 | 0.999677 |
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Target: 5'- cUCCAuac-UUUUCG-GUUUUUCCAUCa -3' miRNA: 3'- -AGGUuuaaAAAGGUgCGAAAAGGUAG- -5' |
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| 15513 | 5' | -41.9 | NC_004001.2 | + | 27375 | 0.67 | 0.99885 |
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Target: 5'- aCCAGGgaaacaCCACGg-UUUCCAUCu -3' miRNA: 3'- aGGUUUaaaaa-GGUGCgaAAAGGUAG- -5' |
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| 15513 | 5' | -41.9 | NC_004001.2 | + | 1410 | 0.7 | 0.9903 |
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Target: 5'- cUCAAGUUUUUCCACuGCUggacgUUUCCc-- -3' miRNA: 3'- aGGUUUAAAAAGGUG-CGA-----AAAGGuag -5' |
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| 15513 | 5' | -41.9 | NC_004001.2 | + | 5768 | 0.7 | 0.986798 |
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Target: 5'- aCCAGGggg-UCCAC-CUUUUCCAa- -3' miRNA: 3'- aGGUUUaaaaAGGUGcGAAAAGGUag -5' |
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| 15513 | 5' | -41.9 | NC_004001.2 | + | 4445 | 0.73 | 0.942936 |
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Target: 5'- cUCCAAGaUUUUCCAUGCacucaUCCAUg -3' miRNA: 3'- -AGGUUUaAAAAGGUGCGaaa--AGGUAg -5' |
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| 15513 | 5' | -41.9 | NC_004001.2 | + | 4465 | 1.12 | 0.010445 |
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Target: 5'- cUCCAAAUUUUUCCACGCUUUUCCAUCc -3' miRNA: 3'- -AGGUUUAAAAAGGUGCGAAAAGGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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