Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15516 | 3' | -54.1 | NC_004001.2 | + | 6025 | 0.66 | 0.635278 |
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Target: 5'- uGCAgaggUCAUgCCCGCCUuCGAGAGGg -3' miRNA: 3'- -UGUauu-GGUGgGGGCGGGuGUUCUUC- -5' |
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| 15516 | 3' | -54.1 | NC_004001.2 | + | 27780 | 0.66 | 0.612202 |
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Target: 5'- -aAUAAUCACaagCCCaCCCGCAAGAAu -3' miRNA: 3'- ugUAUUGGUGg--GGGcGGGUGUUCUUc -5' |
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| 15516 | 3' | -54.1 | NC_004001.2 | + | 23377 | 0.67 | 0.577757 |
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Target: 5'- cACAgggAGCaCACCCCCGaaCGC-GGAAGg -3' miRNA: 3'- -UGUa--UUG-GUGGGGGCggGUGuUCUUC- -5' |
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| 15516 | 3' | -54.1 | NC_004001.2 | + | 20295 | 0.69 | 0.436387 |
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Target: 5'- -aAUGACCAgCCucugaaagCCGCCCaguuGCGAGAAGg -3' miRNA: 3'- ugUAUUGGUgGG--------GGCGGG----UGUUCUUC- -5' |
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| 15516 | 3' | -54.1 | NC_004001.2 | + | 7305 | 0.73 | 0.249875 |
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Target: 5'- cGCuuuGCCcaaCCCCGCCUACAAGAAa -3' miRNA: 3'- -UGuauUGGug-GGGGCGGGUGUUCUUc -5' |
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| 15516 | 3' | -54.1 | NC_004001.2 | + | 9308 | 0.75 | 0.189485 |
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Target: 5'- cGCGUGcccccuCCGCCCCCGCCUgaagACgAAGAAGa -3' miRNA: 3'- -UGUAUu-----GGUGGGGGCGGG----UG-UUCUUC- -5' |
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| 15516 | 3' | -54.1 | NC_004001.2 | + | 4781 | 0.75 | 0.189485 |
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Target: 5'- cACAauUAAUCACCCCCGCCC-CA-GAAa -3' miRNA: 3'- -UGU--AUUGGUGGGGGCGGGuGUuCUUc -5' |
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| 15516 | 3' | -54.1 | NC_004001.2 | + | 9646 | 1.08 | 0.000839 |
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Target: 5'- cACAUAACCACCCCCGCCCACAAGAAGc -3' miRNA: 3'- -UGUAUUGGUGGGGGCGGGUGUUCUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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