miRNA: 3'- gUUUGAGCgC-GUGUU--UGAGGCGg--UCg -5'
miRNA: 3'- -GUUUgAGCGCGUGUuUGAG---------GCGGUCg -5'
miRNA: 3'- guUUGAGCGcGUguu--UG-AGGCGGUCG- -5'
miRNA: 3'- -GUUUGAgCGCGUGu------UUGAGGCGGUCG- -5'
miRNA: 3'- -GUUUGAGCGCGUGUUUGAGGCGGUCG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 15521 | 5' | -52.1 | NC_004001.2 | + | 24144 | 0.66 | 0.752959 |
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Target: 5'- gAGACUCGgGcCGCAAauGCUCUGCaaaAGg -3' miRNA: 3'- gUUUGAGCgC-GUGUU--UGAGGCGg--UCg -5' |
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| 15521 | 5' | -52.1 | NC_004001.2 | + | 32948 | 0.71 | 0.427018 |
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Target: 5'- aCAAGgUUGCGCGCAcGCUCgacaucaugCGCCAGa -3' miRNA: 3'- -GUUUgAGCGCGUGUuUGAG---------GCGGUCg -5' |
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| 15521 | 5' | -52.1 | NC_004001.2 | + | 30245 | 0.72 | 0.416961 |
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Target: 5'- -cGACUCGCcCAacuacGCaUCCGCCAGCa -3' miRNA: 3'- guUUGAGCGcGUguu--UG-AGGCGGUCG- -5' |
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| 15521 | 5' | -52.1 | NC_004001.2 | + | 15071 | 0.75 | 0.269852 |
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Target: 5'- aCAAGCU-GUGUACucccagcAGCUCCGCCAGUc -3' miRNA: 3'- -GUUUGAgCGCGUGu------UUGAGGCGGUCG- -5' |
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| 15521 | 5' | -52.1 | NC_004001.2 | + | 16188 | 1.11 | 0.000805 |
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Target: 5'- gCAAACUCGCGCACAAACUCCGCCAGCc -3' miRNA: 3'- -GUUUGAGCGCGUGUUUGAGGCGGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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