Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1553 | 3' | -52.5 | NC_001347.2 | + | 83827 | 0.66 | 0.994402 |
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Target: 5'- -aGCUGG-CGUUCGgcaUGCUCUUUUUCa -3' miRNA: 3'- caUGACCgGCAGGCa--GUGAGGAAAAG- -5' |
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| 1553 | 3' | -52.5 | NC_001347.2 | + | 184180 | 0.66 | 0.992586 |
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Target: 5'- -gGCUGGCUagagGUCCGUCuuCUUCg--UCg -3' miRNA: 3'- caUGACCGG----CAGGCAGu-GAGGaaaAG- -5' |
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| 1553 | 3' | -52.5 | NC_001347.2 | + | 210591 | 0.66 | 0.991517 |
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Target: 5'- -aGCUGGUa---CGUCGCUCCUUg-- -3' miRNA: 3'- caUGACCGgcagGCAGUGAGGAAaag -5' |
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| 1553 | 3' | -52.5 | NC_001347.2 | + | 128810 | 0.67 | 0.982323 |
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Target: 5'- -cGC-GGCCGUCaCGaCGCUCCg--UCg -3' miRNA: 3'- caUGaCCGGCAG-GCaGUGAGGaaaAG- -5' |
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| 1553 | 3' | -52.5 | NC_001347.2 | + | 157535 | 0.69 | 0.963866 |
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Target: 5'- ---gUGGCCGUCCG-CACgauggCCUUUc- -3' miRNA: 3'- caugACCGGCAGGCaGUGa----GGAAAag -5' |
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| 1553 | 3' | -52.5 | NC_001347.2 | + | 89064 | 0.69 | 0.952844 |
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Target: 5'- uUGCUgcacGGCCGcCCGUUGCUCCa---- -3' miRNA: 3'- cAUGA----CCGGCaGGCAGUGAGGaaaag -5' |
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| 1553 | 3' | -52.5 | NC_001347.2 | + | 195370 | 0.71 | 0.913197 |
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Target: 5'- gGUGuCUGGCUuuaccgCCGUCGCUCCgcgUCg -3' miRNA: 3'- -CAU-GACCGGca----GGCAGUGAGGaaaAG- -5' |
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| 1553 | 3' | -52.5 | NC_001347.2 | + | 114917 | 0.73 | 0.842738 |
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Target: 5'- cGUACUGGCCGcCCG-CGC-CCUUc-- -3' miRNA: 3'- -CAUGACCGGCaGGCaGUGaGGAAaag -5' |
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| 1553 | 3' | -52.5 | NC_001347.2 | + | 199043 | 1.11 | 0.006821 |
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Target: 5'- cGUACUGGCCGUCCGUCACUCCUUUUCg -3' miRNA: 3'- -CAUGACCGGCAGGCAGUGAGGAAAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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