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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 15538 | 3' | -41.8 | NC_004003.1 | + | 149204 | 0.66 | 1 |
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Target: 5'- aCCaAAACAAauaaUCAGA-GAUGGCGg -3' miRNA: 3'- -GGcUUUGUUgaaaAGUCUaCUACUGC- -5' |
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| 15538 | 3' | -41.8 | NC_004003.1 | + | 607 | 1.16 | 0.039322 |
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Target: 5'- aCCGAAACAACUUUUCAGAUGAUGACGa -3' miRNA: 3'- -GGCUUUGUUGAAAAGUCUACUACUGC- -5' |
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| 15538 | 3' | -41.8 | NC_004003.1 | + | 148993 | 1.16 | 0.039322 |
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Target: 5'- aCCGAAACAACUUUUCAGAUGAUGACGa -3' miRNA: 3'- -GGCUUUGUUGAAAAGUCUACUACUGC- -5' |
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| 15538 | 3' | -41.8 | NC_004003.1 | + | 124825 | 0.71 | 0.999996 |
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Target: 5'- gCCGAAGCAAUgagUUCuGAUGuuuGUGAUa -3' miRNA: 3'- -GGCUUUGUUGaa-AAGuCUAC---UACUGc -5' |
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| 15538 | 3' | -41.8 | NC_004003.1 | + | 117888 | 0.68 | 1 |
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Target: 5'- aCGAAAauauUUUUUUAGAUGAUGAa- -3' miRNA: 3'- gGCUUUguu-GAAAAGUCUACUACUgc -5' |
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| 15538 | 3' | -41.8 | NC_004003.1 | + | 396 | 0.66 | 1 |
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Target: 5'- aCCaAAACAAauaaUCAGA-GAUGGCGg -3' miRNA: 3'- -GGcUUUGUUgaaaAGUCUaCUACUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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