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miRNA: 3'- gCGGUgUaCUUCGgCGCCGCCG-CGCA- -5'
miRNA: 3'- gCGgUGuacuUCGGCGCCGCCGCGca -5'
miRNA: 3'- gCGGu----GUACUUCGgCGCCGCCGC-GCa -5'
miRNA: 3'- gCGGUGuACuuCGGCG-CCGCCGCGCa -5'
miRNA: 3'- -GCGGUGUACUUCGGCGCCGCCGCGCA- -5'
Results 101 - 105 of 105 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 1555 | 5' | -61.4 | NC_001347.2 | + | 106229 | 0.75 | 0.277476 |
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Target: 5'- aGCCAgAcGAGGCgGCGGCGGUgGCGUu -3' miRNA: 3'- gCGGUgUaCUUCGgCGCCGCCG-CGCA- -5' |
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| 1555 | 5' | -61.4 | NC_001347.2 | + | 101165 | 0.77 | 0.226466 |
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Target: 5'- aGCgGCuu--GGCCGCGGCGGCGCa- -3' miRNA: 3'- gCGgUGuacuUCGGCGCCGCCGCGca -5' |
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| 1555 | 5' | -61.4 | NC_001347.2 | + | 63810 | 0.77 | 0.221317 |
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Target: 5'- uGCCcaccuCAUGAAGCgGCGGCGGCGaUGg -3' miRNA: 3'- gCGGu----GUACUUCGgCGCCGCCGC-GCa -5' |
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| 1555 | 5' | -61.4 | NC_001347.2 | + | 147897 | 0.79 | 0.175136 |
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Target: 5'- aGCCAU-UGggGCCGUgGGUGGCGCGg -3' miRNA: 3'- gCGGUGuACuuCGGCG-CCGCCGCGCa -5' |
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| 1555 | 5' | -61.4 | NC_001347.2 | + | 197248 | 1.07 | 0.002131 |
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Target: 5'- aCGCCACAUGAAGCCGCGGCGGCGCGUu -3' miRNA: 3'- -GCGGUGUACUUCGGCGCCGCCGCGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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